Six specimens of
lung adenocarcinoma tissues and seven specimens of lung
squamous carcinoma tissues obtained during operation were made into frozen sections and stained by improved H-E
solution. About 1.2 x 10(5) of homogeneous
adenocarcinoma cells and 1.4 x 10(5) of homogeneous lung
squamous carcinoma cells were collected using LCM. Then SELDI profiles based on PBS II(+)SELDI-TOF-MS (
IMAC protein chip) and the data were analyzed by support vector machine (SVM).
RESULTS: Eighty seven differential
protein peaks were found and top ten of them were identified as candidate
biomarkers. The expression levels of 6
proteins among them with the molecular weights of 3333, 3592, 3848, 5036, 5191, and 5211 respectively in the lung squamous
cancer tissues were weaker than those in the
adenocarcinoma tissues, and the expression levels of 4
proteins with the molecular weights of 2505, 4004, 4847, and 11 412 in the lung
squamous carcinoma tissues were stronger than those in the
adenocarcinoma tissues. The expression of the
protein with the molecular weight of 4847 in the squamous
cancer was significantly stronger than that in the
adenocarcinoma (p + 0.032). A discriminatory pattern consisting of 3
proteins with the molecular weights of 4847, 11 412, and 3592 was established with a sensitivity of 100% and a specificity of 100% respectively in separating
adenocarcinoma from
squamous carcinoma.
CONCLUSION: