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Dendritic cell differentiation state and their interaction with NKT cells determine Th1/Th2 differentiation in the murine model of Leishmania major infection.

Abstract
Recent reports demonstrated that dendritic cells (DC) sense inflammatory and microbial signals differently, redefining their classical subdivision into an immature endocytic and a mature Ag-presenting differentiation stage. Although both signals induce DC maturation by up-regulating MHC class II and costimulatory molecules, only TLR signals such as LPS are able to trigger proinflammatory cytokine secretion by DCs, including Th1-polarizing IL-12. Here, we explored the murine Leishmania major infection model to examine the CD4(+) T cell response induced by differentially matured DCs. When partially matured TNF-DCs were injected into BALB/c mice before infection, the mice failed to control L. major infection and developed a Th2 response which was dependent on IL-4Ralpha signaling. In contrast, injections of fully matured LPS+CD40-DCs induced a Th1 response controlling the infection. Pulsing DCs with a lysate of L. major did not affect DC maturation with TNF-alpha or LPS+anti-CD40. When the expression of different Notch ligands on DCs was analyzed, we found increased expression of Th2-promoting Jagged2 in TNF-DCs, whereas LPS+CD40-DCs up-regulated the Th1-inducing Delta4 and Jagged1 molecules. The Th2 polarization induced by TNF-DCs required interaction with CD1d-restricted NKT cells. However, NKT cell activation by L. major lysate-pulsed DCs was not affected by blockade of the endogenous glycolipid, suggesting exchange with exogenous parasite-derived CD1 glycolipid Ag. In sum, the differentiation stage of DCs as well as their interaction with NKT cells determines Th1/Th2 differentiation. These results have generic implications for the understanding of DC-driven Th cell responses and the development of improved DC vaccines against leishmaniasis.
AuthorsCarsten Wiethe, Andrea Debus, Markus Mohrs, Alexander Steinkasserer, Manfred Lutz, André Gessner
JournalJournal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 180 Issue 7 Pg. 4371-81 (Apr 01 2008) ISSN: 0022-1767 [Print] United States
PMID18354157 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, CD1
  • Antigens, CD1d
  • Cytokines
  • Ligands
  • Receptors, Notch
Topics
  • Animals
  • Antigens, CD1 (metabolism)
  • Antigens, CD1d
  • Cell Differentiation (immunology)
  • Cytokines (immunology)
  • Dendritic Cells (cytology, immunology)
  • Disease Models, Animal
  • Leishmania major (immunology)
  • Leishmaniasis (immunology, parasitology)
  • Ligands
  • Mice
  • Receptors, Notch (metabolism)
  • T-Lymphocytes, Regulatory (immunology, metabolism)
  • Th1 Cells (cytology, immunology, metabolism)
  • Th2 Cells (cytology, immunology, metabolism)

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