Kinetics and determinants of the changes of CC16, a lung secretory protein in a rat model of toxic lung injury.

Abstract

PURPOSE:

To investigate the kinetics and the determinants of the changes of the Clara cell 16 kDa protein (CC16) as a marker of lung injury following exposure to different toxicants.

METHODS:

Rats were treated with 4-Ipomeanol (IPO), alpha-naphtylthiourea (ANTU), sodium chromate (Na(2)CrO(4)) or paraquat-(PQ). The changes of CC16, total protein, albumin, and cystatin-C were determined on BALF supernatan, and CC16, cystatin-C and creatinine levels were also determined in serum.

RESULTS:

Bronchiolar insult due to IPO or ANTU resulted in an initial transient increase of serum CC16, parallel to that of albumin in BALF, and a subsequent reduction in both BALF and serum. A slight reduction of CC16 in BALF was already apparent one hour following treatment with PQ. In the serum, CC16 increased to 400% of basal value. With PQ and Na(2)CrO(4), the elevation of CC16 was mainly determined by the degree of renal impairment.

• Authors: Philippe Hantson, Alfred Bernard, Cedric Hermans
• Journal: Clinical toxicology (Philadelphia, Pa.) (Clin Toxicol (Phila)) Vol. 46 Issue 3 Pg. 230-8 (Mar 2008) ISSN: 1556-3650 [Print] England
• PMID: 18344105 (Publication Type: Journal Article)

Chemical References

• Albumins
• Biomarkers
• Cystatins
• Scgb1a1 protein, rat
• Uteroglobin

Topics

• Albumins (metabolism)
• Animals
• Biomarkers
• Bronchoalveolar Lavage Fluid (chemistry)
• Calibration
• Cystatins (metabolism)
• Female
• Kinetics
• Lung Diseases (chemically induced, metabolism)
• Rats
• Rats, Sprague-Dawley
• Uteroglobin (metabolism)