Maximal inflammatory response benefits syngeneic skin graft acceptance.

Abstract

OBJECTIVE AND DESIGN:

We investigated the influence of acute inflammation in skin isograft acceptance.

METHODS:

Two mouse lines selected for maximal (AIRMAX) or minimal inflammatory response (AIRMIN) were transplanted with syngeneic skin. Cellular infiltrates and cytokine production were measured 1, 3, 7 or 14 days post-transplantation. The percentage of CD4+CD25+Foxp3+ cells in the lymph nodes was also evaluated.

RESULTS:

Grafts were totally accepted in 100% of AIRMAX and in 26% of AIRMIN mice. In the latter, partial acceptance was observed in 74% of the animals. Emigrated cells were basically PMN and were enhanced in AIRMAX transplants. IL-10 production by graft infiltrating cells showed no interline differences. IFN-gamma was increased in AIRMIN grafts at day 14 and lower percentages of CD4+CD25+Foxp3+ cells in the lymph nodes were observed in these mice.

CONCLUSIONS:

Our data suggest that differences in graft acceptance might be due to a lack of appropriate regulation of the inflammatory response in AIRMIN mice compromising the self/non-self recognition.

• Authors: R Larocca, I Marguti, W Cabrera, O Garcia Ribeiro, L V Rizzo, L Vieira de Moraes
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 57 Issue 4 Pg. 171-7 (Apr 2008) ISSN: 1023-3830 [Print] Switzerland
• PMID: 18344060 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CD4 Antigens
• Forkhead Transcription Factors
• Foxp3 protein, mouse
• Interleukin-2 Receptor alpha Subunit
• Tumor Necrosis Factor-alpha
• Interleukin-10
• Interferon-gamma

Topics

• Animals
• CD4 Antigens (metabolism)
• Forkhead Transcription Factors (metabolism)
• Graft Survival (physiology)
• Inflammation (metabolism, pathology)
• Interferon-gamma (metabolism)
• Interleukin-10 (metabolism)
• Interleukin-2 Receptor alpha Subunit (metabolism)
• Lymph Nodes (metabolism, pathology)
• Macrophages (metabolism, pathology)
• Mice
• Mice, Inbred Strains
• Models, Animal
• Neutrophils (metabolism, pathology)
• Skin Transplantation (pathology, physiology)
• Transplantation, Isogeneic (pathology)
• Tumor Necrosis Factor-alpha (metabolism)