Abstract |
Protective immunity against intracellular pathogen Mycobacterium leprae is dependent on the activation of T cells. Repeated stimulation of T cells by M. leprae antigens MLCwA (M. leprae total cell wall antigen) and ManLAM ( mannose capped lipoarabinomannan) may lead to apoptosis in leprosy patients. In the present study, inhibition of the Fas-induced apoptosis of peripheral blood mononuclear cells of leprosy patients was investigated using above M. leprae antigen(s), in combination with immunomodulators murabutide (MB) and a Trat peptide in particulate form ( liposome). Incubation of the cells with particulate mode of antigen presentation led to both decreased percentage of propidium iodide (PI) positive cells and T cells expressing Fas-FasL, as well as decreased caspase-8/-3 activities in the lepromatous patients, thereby inhibiting apoptosis, while converse was true with stimulation with soluble antigen. Concurrently, there was an upregulation of antiapoptotic protein Bcl-X(L) in the lepromatous patients, thereby inhibiting apoptosis. Thus, the liposomal formulation of antigen promoted proliferation of anergized T cell by inhibiting apoptosis through decreased expression of death receptors and caspase activities and increased expression of anti-apoptotic protein Bcl-X(L) in these patients.
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Authors | Vineeta Chattree, Neena Khanna, Vandana Bisht, D N Rao |
Journal | Indian journal of biochemistry & biophysics
(Indian J Biochem Biophys)
Vol. 44
Issue 5
Pg. 386-93
(Oct 2007)
ISSN: 0301-1208 [Print] India |
PMID | 18341215
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Bacterial Outer Membrane Proteins
- Escherichia coli Proteins
- Liposomes
- TraT protein, E coli
- fas Receptor
- Acetylmuramyl-Alanyl-Isoglutamine
- murabutide
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Topics |
- Acetylmuramyl-Alanyl-Isoglutamine
(administration & dosage, analogs & derivatives)
- Adjuvants, Immunologic
(administration & dosage)
- Adult
- Apoptosis
(drug effects, immunology)
- Bacterial Outer Membrane Proteins
(administration & dosage)
- Cells, Cultured
- Drug Delivery Systems
(methods)
- Escherichia coli Proteins
(administration & dosage)
- Female
- Humans
- Leprosy
(immunology, pathology)
- Leukocytes, Mononuclear
(drug effects, immunology)
- Liposomes
(chemistry)
- Male
- Middle Aged
- fas Receptor
(immunology)
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