HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

[Binding of SSB-protein from Ehrlich ascites carcinoma cells with DNA and polyribonucleotides].

Abstract
Binding of SSB-protein from Ehrlich ascites tumor to ssDNA from M13 phage leads to its compactization. The structure of the complex at the protein/DNA ratios far from the saturation level looks like "beads-on the string". DNA that was fully saturated with protein forms collapsed globular structure. Binding of the protein to the dsDNA from phage lambda increases its flexibility and decreases the coil dimensions; no "beads-on the string" structure are seen. The protein possess slight destabilizing effect on hairpin helices of M13DNA. Competition studies demonstrate that the binding properties of protein with polyribonucleotide lattices and DNA's decrease in ranking as follows: poly(rG) greater than or equal to poly(rI) greater than or equal to ssDNA greater than dsDNA greater than poly(rA) congruent to approximately poly(rU). Thus SSB-protein from Ehrlich ascites tumor differs significantly from its presumed prokaryotic analogs.
AuthorsV A Tronov, V A Zaĭtsev, D I Chernyĭ, A N Koterov, I V Filippovich
JournalMolekuliarnaia biologiia (Mol Biol (Mosk)) 1991 Jan-Feb Vol. 25 Issue 1 Pg. 212-22 ISSN: 0026-8984 [Print] Russia (Federation)
Vernacular TitleSviazyvanie SSB-belka iz kletok astsitnoĭ kartsinomy érlikha s DNK i poliribonukleotidami.
PMID1832738 (Publication Type: English Abstract, Journal Article)
Chemical References
  • DNA, Single-Stranded
  • DNA, Viral
  • DNA-Binding Proteins
Topics
  • Animals
  • Bacteriophage lambda (metabolism)
  • Bacteriophages (metabolism)
  • Carcinoma, Ehrlich Tumor (metabolism)
  • DNA, Single-Stranded (metabolism, ultrastructure)
  • DNA, Viral (metabolism, ultrastructure)
  • DNA-Binding Proteins (metabolism, ultrastructure)
  • Microscopy, Electron
  • Osmolar Concentration

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: