Abstract |
We investigated the anti- tumor effect of human interferon-beta (HuIFN-beta) against malignant melanoma. In vitro study revealed that HuIFN-beta not only inhibited proliferation of melanoma cells (seven cell lines: MM-AN, MM-BP, MM-LH, MM-RU, PM-WK, RPM-EP, RPM-MC) but also induced apoptosis in a dose dependent fashion, though the sensitivity to HuIFN-beta was different among cell lines. In addition, we administered HuIFN-beta into cutaneous metastatic lesions of melanoma and evaluated clinical and histopathological effects. Although the size of the metastatic cutaneous lesion did not change by the intralesional injection of HuIFN-beta, histopathological examination revealed apoptotic changes of melanoma cells along with dense lymphohistiocytic infiltration. The present study confirmed direct and indirect inhibitory effects of HuIFN-beta on human melanoma cells and suggests that local higher concentration of HuIFN-beta is needed to eradicate melanoma lesions.
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Authors | Hitomi Kubo, Atsuko Ashida, Kazuhiko Matsumoto, Toshiro Kageshita, Akifumi Yamamoto, Toshiaki Saida |
Journal | Archives of dermatological research
(Arch Dermatol Res)
Vol. 300
Issue 6
Pg. 297-301
(Jul 2008)
ISSN: 1432-069X [Electronic] Germany |
PMID | 18324409
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Apoptosis
(drug effects, immunology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Dose-Response Relationship, Drug
- Down-Regulation
- Humans
- Immunotherapy
- Injections, Intralesional
- Interferon-beta
(pharmacology, therapeutic use)
- Melanoma
(drug therapy, pathology)
- Neoplasm Metastasis
- Skin Neoplasms
(drug therapy, pathology)
- Vaccination
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