Abstract | OBJECTIVE: METHODS:
Lung cancer SPC-A-1 cells were treated with 2.5, 5 and 10 mg/L for 48 h,and the cells were treated with 2.5 mg/L DDP as positive control. The inhibitory rat was evaluated by MTT assay. Apoptotic rate was determined by TUNEL method. The expression of survivin and caspase-3 mRNA were detected by semi-quantitive RT-PCR. RESULTS: Compared with control group, the inhibitory rate was increased obviously (P < 0.001), the apoptotic rate was increased markedly (P < 0.01), the expression of caspase-3 mRNA was increased significantly (P < 0.05 or P < 0.01), while survivin mRNA was decreased markedly (P < 0.05 or P < 0.01) in SBE groups. CONCLUSION: SBE can induce apoptosis of SPCA-1 cells. The molecular mechanism may be related to up-regulating expression of caspase-3 and down-regulating expression of survivin genes.
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Authors | Peng-ya Wei, Hong-qin Pu, Xing Wei, Chao-gan Li, Song Nong |
Journal | Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
(Zhong Yao Cai)
Vol. 30
Issue 10
Pg. 1270-3
(Oct 2007)
ISSN: 1001-4454 [Print] China |
PMID | 18300501
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- BIRC5 protein, human
- Drugs, Chinese Herbal
- Inhibitor of Apoptosis Proteins
- Microtubule-Associated Proteins
- Neoplasm Proteins
- RNA, Messenger
- Survivin
- Caspase 3
- Cisplatin
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects, genetics)
- Caspase 3
(genetics)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cisplatin
(pharmacology)
- Dose-Response Relationship, Drug
- Drugs, Chinese Herbal
(pharmacology)
- Gene Expression
(drug effects)
- Humans
- In Situ Nick-End Labeling
- Inhibitor of Apoptosis Proteins
- Lung Neoplasms
(genetics, pathology)
- Microtubule-Associated Proteins
(genetics)
- Neoplasm Proteins
(genetics)
- Plants, Medicinal
(chemistry)
- RNA, Messenger
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Scutellaria
(chemistry)
- Survivin
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