[Apoptosis-inducing effect of Scutellaria barbata extract on human lung cancer SPC-A-1 cells and the expression of apoptosis associated genes].

Abstract

OBJECTIVE:

To explore the apoptosis-inducing effect of Scutellaria barbata extract (SBE) and the expression of apoptosis associated genes survivin and caspase-3 on human lung cancer SPC-A-1 cells.

METHODS:

Lung cancer SPC-A-1 cells were treated with 2.5, 5 and 10 mg/L for 48 h,and the cells were treated with 2.5 mg/L DDP as positive control. The inhibitory rat was evaluated by MTT assay. Apoptotic rate was determined by TUNEL method. The expression of survivin and caspase-3 mRNA were detected by semi-quantitive RT-PCR.

RESULTS:

Compared with control group, the inhibitory rate was increased obviously (P < 0.001), the apoptotic rate was increased markedly (P < 0.01), the expression of caspase-3 mRNA was increased significantly (P < 0.05 or P < 0.01), while survivin mRNA was decreased markedly (P < 0.05 or P < 0.01) in SBE groups.

CONCLUSION:

SBE can induce apoptosis of SPCA-1 cells. The molecular mechanism may be related to up-regulating expression of caspase-3 and down-regulating expression of survivin genes.

• Authors: Peng-ya Wei, Hong-qin Pu, Xing Wei, Chao-gan Li, Song Nong
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 30 Issue 10 Pg. 1270-3 (Oct 2007) ISSN: 1001-4454 [Print] China
• PMID: 18300501 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• BIRC5 protein, human
• Drugs, Chinese Herbal
• Inhibitor of Apoptosis Proteins
• Microtubule-Associated Proteins
• Neoplasm Proteins
• RNA, Messenger
• Survivin
• Caspase 3
• Cisplatin

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects, genetics)
• Caspase 3 (genetics)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cisplatin (pharmacology)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (pharmacology)
• Gene Expression (drug effects)
• Humans
• In Situ Nick-End Labeling
• Inhibitor of Apoptosis Proteins
• Lung Neoplasms (genetics, pathology)
• Microtubule-Associated Proteins (genetics)
• Neoplasm Proteins (genetics)
• Plants, Medicinal (chemistry)
• RNA, Messenger (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Scutellaria (chemistry)
• Survivin