Abstract | PURPOSE: METHODS: Ethical approval and informed consent were obtained. Mucosal biopsies from 9 patients with familial adenomatous polyposis and 12 with ulcerative colitis were obtained. Sulphomucin levels were assessed by using the high iron- diamine stain. Mucous gel layer composition was correlated with villous height, crypt depth, and total mucosal thickness. Mucous gel layer composition was correlated with acute and chronic inflammatory infiltrates. Colonization by a panel of seven bacterial species (including sulphate reducing bacteria) was established and correlated with sulphomucin levels. RESULTS: High- iron- diamine positivity (i.e., sulphomucin expression) was greater in ulcerative colitis pouch mucous gel (2.083 +/- 0.5 vs. 0.556 +/- 0.4, P = 0.003). Sulphomucin expression correlated with reduced crypt depth, villous height, and total mucosal thickness. In the ulcerative colitis group, chronic inflammatory infiltrate scores were significantly greater for high- iron- diamine-positive patients. Colonization by sulphate reducing bacteria was increased in high- iron- diamine-positive patients. CONCLUSIONS:
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Authors | Niamh Bambury, J Calvin Coffey, John Burke, H Paul Redmond, William O Kirwan |
Journal | Diseases of the colon and rectum
(Dis Colon Rectum)
Vol. 51
Issue 5
Pg. 561-7
(May 2008)
ISSN: 1530-0358 [Electronic] United States |
PMID | 18299930
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Adenomatous Polyposis Coli
(metabolism, surgery)
- Biopsy
- Colitis, Ulcerative
(metabolism, surgery)
- Colonic Pouches
(microbiology)
- Female
- Humans
- Male
- Mucins
(metabolism)
- Statistics, Nonparametric
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