Abstract | BACKGROUND: METHODS AND RESULTS: We show here that circulating renalase lacks significant amine oxidase activity under basal conditions (prorenalase) but that a brief surge of epinephrine lasting <2 minutes causes renalase activity to increase from 48+/-18 to 2246+/-98 arbitrary units (n=3; P<0.002). Enzyme activation is detectable within 30 seconds and sustained for at least 60 minutes. Analysis of epinephrine-mediated hemodynamic changes in normotensive rats indicates that prorenalase becomes maximally activated when systolic pressure increases by >5 mm Hg. The catecholamine surge also leads to a 2.8-fold increase in plasma renalase concentration. Cultured cells exposed to dopamine upregulate steady-state renalase gene expression by >10-fold. The time course of prorenalase activation is abnormal in rats with chronic kidney disease. CONCLUSIONS:
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Authors | Guoyong Li, Jianchao Xu, Peili Wang, Heino Velazquez, Yanyan Li, Yanling Wu, Gary V Desir |
Journal | Circulation
(Circulation)
Vol. 117
Issue 10
Pg. 1277-82
(Mar 11 2008)
ISSN: 1524-4539 [Electronic] United States |
PMID | 18299506
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Catecholamines
- Monoamine Oxidase
- renalase
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Topics |
- Animals
- Catecholamines
(physiology)
- Hemodynamics
- Humans
- Kidney
(enzymology)
- Monoamine Oxidase
(metabolism)
- Myocardium
(enzymology)
- Rats
- Rats, Sprague-Dawley
- Renal Insufficiency, Chronic
(enzymology)
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