Inhibition of DNA helicase, ATPase and DNA-binding activities of E. coli RecQ helicase by chemotherapeutic agents.

Abstract

RecQ helicases play an essential role in maintaining genetic integrity in all organisms from Escherichia coli to humans. Defects to these enzymes are responsible for three distinct human diseases: Werner syndrome, Bloom syndrome and Rothmund-Thomson syndrome. All three diseases are characterized by a predisposition to cancer due to increased genomic instability. Previous studies on the effects of non-covalent DNA modifications on the catalytic activity of purified Werner and Bloom DNA helicases have shown that both enzymes have similar sensitivity profiles to these DNA-binding agents and are most strongly inhibited by the minor groove binder distamycin A. In this study, we show that the sensitivity profiles of E. coli RecQ to a number of DNA-binding ligands are different to those observed for WRN and Bloom helicases. These observations may give insights into the differences in molecular mechanisms underlying efficient motor function of RecQ helicases.

• Authors: Bo Zhang, Ai-hua Zhang, Lei Chen, Xu Guang Xi
• Journal: Journal of biochemistry (J Biochem) Vol. 143 Issue 6 Pg. 773-9 (Jun 2008) ISSN: 0021-924X [Print] England
• PMID: 18296713 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• Distamycins
• Enzyme Inhibitors
• Intercalating Agents
• Topoisomerase I Inhibitors
• stallimycin
• DNA
• Adenosine Triphosphatases
• DNA Helicases
• RecQ Helicases

Topics

• Adenosine Triphosphatases (antagonists & inhibitors, genetics, metabolism)
• Antiviral Agents (pharmacology)
• DNA (genetics, metabolism)
• DNA Helicases (antagonists & inhibitors, genetics, metabolism)
• Distamycins (pharmacology)
• Enzyme Inhibitors (pharmacology)
• Escherichia coli (enzymology)
• Intercalating Agents (pharmacology)
• RecQ Helicases (antagonists & inhibitors, genetics, metabolism)
• Topoisomerase I Inhibitors