Altered beta-1,4-galactosyltransferase I expression during early inflammation after spinal cord contusion injury.

Abstract

Post-traumatic inflammation has been implicated in secondary tissue damage after spinal cord injury (SCI). beta-1,4-Galactosyltransferase I (beta-1,4-GalT-I) is a key inflammatory mediator that plays a critical role in the initiation and maintenance of inflammatory reaction in diseases. The aim of the current study was to investigate whether beta-1,4-GalT-I is expressed in SCI. Spinal cord contusion model was established in adult rats. Real-time PCR and Western blot analysis were used to detect the spatio-temporal expression of beta-1,4-GalT-I after SCI. Lectin-fluorescent staining with RCA-I was used to detect the galactosylation of the membrane glycoproteins. The interaction and colocalization between beta-1,4-GalT-I and E-selectin in the injured spinal cords were also assessed by immunoprecipitation of E-selectin and double immunofluorescent staining, respectively. Real-time PCR revealed that beta-1,4-GalT-I mRNA reached the peak at 1d after spinal cord contusion. In situ hybridization indicated that beta-1,4-GalT-I mRNA was mainly distributed in the local inflammatory cells, adjacent to the center of injury. Double immunofluorescent staining showed that beta-1,4-GalT-I mostly overlapped with ED1-positive macrophages 1d after SCI, partly colocalized with microglia, neutrophils and a few with oligodendrocytes and astrocytes. The result of Lectin-fluorescent staining with RCA-I was similar to that of double immunofluorescent staining. Terminal galactosylation of E-selectin underwent obvious changes between sham and 3d after SCI by immunoprecipitation of E-selectin. Thus, the transient expression of high levels of beta-1,4-GalT-I may provide new insight into the early inflammation after SCI.

• Authors: Shuqiong Niu, Min Fei, Chun Cheng, Meijuan Yan, Shangfeng Gao, Mengling Chen, Haibo Wang, Xin Li, Xiaowei Yu, Ji Qian, Jing Qin, Jian Zhao, Jianxin Gu, Aiguo Shen
• Journal: Journal of chemical neuroanatomy (J Chem Neuroanat) Vol. 35 Issue 3 Pg. 245-56 (May 2008) ISSN: 0891-0618 [Print] Netherlands
• PMID: 18294815 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• E-Selectin
• Nerve Tissue Proteins
• RNA
• Galactosyltransferases
• beta-1,4-galactosyltransferase I

Topics

• Animals
• Blotting, Western
• Contusions (complications, enzymology)
• E-Selectin (biosynthesis, genetics)
• Fluorescent Antibody Technique
• Galactosyltransferases (biosynthesis, genetics)
• Immunohistochemistry
• Immunoprecipitation
• In Situ Hybridization
• Inflammation (enzymology, etiology)
• Male
• Nerve Tissue Proteins (biosynthesis, genetics)
• RNA (biosynthesis)
• Rats
• Rats, Sprague-Dawley
• Reverse Transcriptase Polymerase Chain Reaction
• Spinal Cord Injuries (complications, enzymology)