Measurement of oxygenation at the site of stem cell therapy in a murine model of myocardial infarction.

Abstract

We have developed a noninvasive EPR (electron paramagnetic resonance) oximetry, based on a new class of oxygen-sensing nano-particulate probe (LiNc-BuO), for simultaneous monitoring of stem-cell therapy and in situ oxygenation (partial pressure of oxygen, pO2) in a mouse model of acute myocardial infarction (AMI). AMI was induced by a permanent occlusion of left-anterior-descending (LAD) coronary artery. Skeletal myoblast (SM) cells were used for therapy. The oximetry probe was implanted in the mid-ventricular region using a needle. Tissue histological studies after 3 weeks of implantation of the probe revealed significant fibrosis, which was solely due to the needle track and not due to the probe particles. The feasibility of long-term monitoring of pO2 was established in control (non-infarct) group of hearts (> 3 months; pO2 = 15.0 +/- 1.2 mmHg,). A mixture of the probe with/without SM cells (1 x 10(5)) was implanted as a single injection in the infarcted region and the myocardial tissue pO2 at the site of cell therapy was measured for 4 weeks. The pO2 was significantly higher in infarcted hearts treated with SM cells (pO2 = 3.5 +/- 0.9 mmHg) compared to untreated hearts (pO2 = 1.6 +/- 0.7 mmHg). We have demonstrated, for the first time, the feasibility of monitoring pO2 in mouse hearts after stem cell therapy.

• Authors: Mahmood Khan, Vijay Kumar Kutala, Sheik Wisel, Simi M Chacko, M Lakshmi Kuppusamy, Pawel Kwiatkowski, Periannan Kuppusamy
• Journal: Advances in experimental medicine and biology (Adv Exp Med Biol) Vol. 614 Pg. 45-52 ( 2008) ISSN: 0065-2598 [Print] United States
• PMID: 18290313 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Culture Media
• Oxygen

Topics

• Animals
• Cell Culture Techniques
• Cells, Cultured
• Culture Media
• Disease Models, Animal
• Electron Spin Resonance Spectroscopy (methods)
• Feasibility Studies
• Hindlimb
• Male
• Mice
• Mice, Inbred C57BL
• Myoblasts, Skeletal (metabolism, transplantation)
• Myocardial Infarction (etiology, metabolism, pathology, physiopathology)
• Myocardium (metabolism, pathology)
• Oximetry
• Oxygen (analysis)
• Oxygen Consumption
• Partial Pressure
• Stem Cell Transplantation
• Time Factors