ESCRT factors restrict mycobacterial growth.

Abstract

Nearly 1.7 billion people are infected with Mycobacterium tuberculosis. Its ability to survive intracellularly is thought to be central to its success as a pathogen, but how it does this is poorly understood. Using a Drosophila model of infection, we identify three host cell activities, Rab7, CG8743, and the ESCRT machinery, that modulate the mycobacterial phagosome. In the absence of these factors the cell no longer restricts growth of the non-pathogen Mycobacterium smegmatis. Hence, we identify factors that represent unique vulnerabilities of the host cell, because manipulation of any one of them alone is sufficient to allow a nonpathogenic mycobacterial species to proliferate. Furthermore, we demonstrate that, in mammalian cells, the ESCRT machinery plays a conserved role in restricting bacterial growth.

• Authors: Jennifer A Philips, Maura C Porto, Hui Wang, Eric J Rubin, Norbert Perrimon
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 105 Issue 8 Pg. 3070-5 (Feb 26 2008) ISSN: 1091-6490 [Electronic] United States
• PMID: 18287038 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• DNA-Binding Proteins
• Endosomal Sorting Complexes Required for Transport
• Multiprotein Complexes
• Transcription Factors
• Tsg101 protein
• Vesicular Transport Proteins
• rab7 GTP-Binding Proteins
• rab GTP-Binding Proteins

Topics

• Animals
• Cell Line
• DNA-Binding Proteins (genetics)
• Drosophila
• Endosomal Sorting Complexes Required for Transport
• Endosomes (metabolism)
• Microscopy, Fluorescence
• Multiprotein Complexes (genetics, metabolism)
• Mycobacterium (growth & development, pathogenicity)
• Mycobacterium Infections (genetics, metabolism)
• RNA Interference
• Species Specificity
• Transcription Factors (genetics)
• Vesicular Transport Proteins (genetics)
• Virulence
• rab GTP-Binding Proteins (genetics)
• rab7 GTP-Binding Proteins