| Abstract | BACKGROUND: In gluten-sensitive enteropathy, antitissue transglutaminase antibodies are synthesized in the duodenum. AIM: To compare the diagnostic yield of these autoantibodies in cultured duodenal biopsies, duodenal aspirate and serum. METHODS: Patients (n = 315, 135 female, 180 male; age: 37.3 +/- 1.1 years) referred for duodenal biopsies, were recruited and HLA-DQ2/DQ8 haplotyped. Histological measurements were made from duodenal biopsies and cultured duodenal biopsies were used for antitissue transglutaminase antibodies analysis by enzyme-linked immunosorbent assay. Duodenal aspirate was collected in a subgroup of 81 patients. Patients were classified, according to their histology, response to a gluten-free diet and DQ2/DQ8 status, as definite, likely or nongluten-sensitive enteropathy. RESULTS: Histology was normal in 59% of patients; 28% had lymphocytic enteritis, 1% had crypt hyperplasia and 13% showed atrophy. In Marsh III patients, there was complete agreement between duodenal and serological antitissue transglutaminase antibodies measurements. Marsh I patients showed a slight antitissue transglutaminase antibodies sensitivity improvement in cultured duodenal biopsy compared to serum in definite (22% vs. 19%) and likely gluten-sensitive enteropathy (20% vs. 14%) patients. Combined serum and cultured duodenal biopsy antitissue transglutaminase antibodies assessment increased serological sensitivity from 19% to 30% in Marsh I patients. CONCLUSION: Duodenal antitissue transglutaminase antibodies detection improves serological determination sensitivity in Marsh I patients, providing diagnostic value and therapeutic impact. |
| Authors | R Santaolalla, F Fernández-Bañares, R Rodríguez, M Alsina, M Rosinach, M Mariné, C Farré, A Salas, M Forné, C Loras, J Espinós, J M Viver, M Esteve
(Affiliation: Department of Gastroenterology, Hospital Mútua de Terrassa, Fundació per la Recerca Mútua de Terrassa, Barcelona, Spain.)
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| Journal | Alimentary pharmacology & therapeutics
(Aliment Pharmacol Ther)
Vol. 27
Issue 9
Pg. 820-9
(May 2008)
ISSN: 1365-2036 England |
| PMID | 18284655
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Autoantibodies
- Biological Markers
- HLA-DQ Antigens
- HLA-DQ2 antigen, human
- Transglutaminases
|
| Topics |
- Adult
- Autoantibodies
(immunology)
- Biological Markers
(blood)
- Celiac Disease
(diagnosis, immunology)
- Duodenum
(enzymology)
- Female
- HLA-DQ Antigens
(immunology)
- Humans
- Male
- Transglutaminases
(immunology)
|
