[Complex of recombinant heat shock protein with lipopolysaccharide induces rapid protection of mice against Salmonella typhimurium and avirulent for humans avian influenza virus H5N2].

Abstract

Protective effect of immunization with heat shock protein (HSP) against bacterial and viral infections in mice was studied. Recombinant HSP 70 kDa of Mycobacterium tuberculosis contaminated with lypopolysaccharide (0.185 mcg/ml) was used for experiments. One intraperitoneal injection of 100 or 400 mcg of HSP induced rapid protection against intraperitoneal challen e with 125 LD50 of Salmonella typhimurium (on 3rd-6th day) and against intranasal challenge with 10 LD50 of avirulent for humans avian influenza virus H5N2 (A/ mallard/Pennsylvania/10218/84) (on 5th-8th day). Three daily injections with 10 mcg of HSP induced rapid, significant and long-term protection against S. typhimurium. Immunization with HSP protected 100% of mice during 3 days after the challenge, 50% of immunized animals survived during 21 days (duration of the study). All nonimmunized mice died on 6th day.

• Authors: I B Semenova, D S Vorob'ev, P G Sveshnikov, E A Kurbatova
• Journal: Zhurnal mikrobiologii, epidemiologii i immunobiologii (Zh Mikrobiol Epidemiol Immunobiol) 2007 Nov-Dec Issue 6 Pg. 54-7 ISSN: 0372-9311 [Print] Russia (Federation)
• PMID: 18283735 (Publication Type: English Abstract, Journal Article)

Chemical References

• Bacterial Proteins
• Heat-Shock Proteins
• Lipopolysaccharides
• Recombinant Proteins
• Vaccines, Combined
• Vaccines, Synthetic

Topics

• Animals
• Bacterial Proteins (administration & dosage, chemistry)
• Heat-Shock Proteins (administration & dosage, chemistry)
• Immunization Schedule
• Influenza A Virus, H5N2 Subtype
• Injections, Intraperitoneal
• Lipopolysaccharides (administration & dosage)
• Mice
• Molecular Weight
• Mycobacterium tuberculosis (chemistry)
• Orthomyxoviridae Infections (prevention & control)
• Recombinant Proteins (administration & dosage, chemistry, metabolism)
• Salmonella Infections, Animal (prevention & control)
• Salmonella typhimurium
• Vaccination
• Vaccines, Combined (administration & dosage, chemistry)
• Vaccines, Synthetic (administration & dosage, chemistry)