Abstract | BACKGROUND & OBJECTIVE: METHODS:
After treatment of MSC, the proliferation and apoptosis of MDA-MB-231 cells were detected with light microscope and Cell Counting Kit-8 (CCK-8), cell cycle and apoptosis were determined by flow cytometry, malignant phenotype was determined by soft agarose growth assay, and the expression of MMP-2 was determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The concentration of MMP-2 protein in culture supernatant was measured with enzyme-linked immunosorbent assay (ELISA). RESULTS: MSC induced S phase arrest and apoptosis, inhibited the proliferation and colony formation of MDA-MB-231 cells. When treated with 50 mumol/L MSC for 48 h and 72 h, the mRNA levels of MMP-2 were up-regulated by 32.2% and 47.1%, whereas its protein levels were down-regulated by 42.4% and 50.8%, its concentrations in culture supernatant were down-regulated by 56.7% and 75.2%. When treated with 100 mumol/L MSC for 48 h and 72 h, the mRNA levels of MMP-2 were up-regulated by 52.6% and 61.3%, whereas its protein levels were down-regulated by 72.9% and 81.4%, and its concentrations in culture supernatant were down-regulated by 68.5% and 80.9%. CONCLUSIONS: MSC could inhibit proliferation, and induce apoptosis and S phase arrest in MDA-MB-231 cells. MSC could also up-regulate the mRNA level of MMP-2 while down-regulate its protein level and secretion in the cells.
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Authors | Juan Wang, Nan-Lin Jiao, Jie Zheng |
Journal | Ai zheng = Aizheng = Chinese journal of cancer
(Ai Zheng)
Vol. 27
Issue 2
Pg. 119-25
(Feb 2008)
China |
PMID | 18279606
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Anticarcinogenic Agents
- Organoselenium Compounds
- RNA, Messenger
- Selenocysteine
- MMP2 protein, human
- Matrix Metalloproteinase 2
- Cysteine
- selenomethylselenocysteine
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Topics |
- Anticarcinogenic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Breast Neoplasms
(drug therapy, enzymology, pathology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cysteine
(analogs & derivatives, pharmacology)
- Female
- Humans
- Matrix Metalloproteinase 2
(analysis, genetics)
- Organoselenium Compounds
(pharmacology)
- RNA, Messenger
(analysis)
- S Phase
(drug effects)
- Selenocysteine
(analogs & derivatives)
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