Abstract |
The adenovirus E1A protein and tax protein (Tax) of human T-cell leukemia virus-1 (HTLV-1) are transcriptional regulators that do not bind to DNA directly. The ATF sites/CRE ( cyclic AMP response element) of the adenovirus E4 promoter and the long terminal repeat of HTLV-1 have been shown to be required for E1A and Tax inducibility, respectively. Using the c-Myb-CRE-BP1 fusion protein, it was shown that CRE-BP1, which could bind to the ATF sites/CRE, mediated the E1A-induced trans-activation. For this activation, the N-terminal portion of CRE-BP1, which contained the putative metal finger structure, was essential but not sufficient. In contrast, the trans-activation induced by HTLV-1 Tax was not mediated by CRE-BP1. These results strongly suggested that E1A activates transcription through interaction with CRE-BP1, but another CRE- binding protein participates in the Tax-induced trans-activation.
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Authors | T Maekawa, S Matsuda, J Fujisawa, M Yoshida, S Ishii |
Journal | Oncogene
(Oncogene)
Vol. 6
Issue 4
Pg. 627-32
(Apr 1991)
ISSN: 0950-9232 [Print] England |
PMID | 1827668
(Publication Type: Journal Article)
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Chemical References |
- Adenovirus Early Proteins
- Cyclic AMP Response Element-Binding Protein
- DNA-Binding Proteins
- Gene Products, tax
- Oncogene Proteins, Viral
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-myb
- Transcription Factors
- Viral Fusion Proteins
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Topics |
- Adenovirus Early Proteins
- Chromosome Mapping
- Cyclic AMP Response Element-Binding Protein
- DNA-Binding Proteins
(pharmacology)
- Gene Expression Regulation, Neoplastic
- Gene Products, tax
- In Vitro Techniques
- Oncogene Proteins, Viral
- Plasmids
- Promoter Regions, Genetic
- Proto-Oncogene Proteins
(pharmacology)
- Proto-Oncogene Proteins c-myb
- Transcription Factors
- Transcriptional Activation
(drug effects)
- Transfection
- Viral Fusion Proteins
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