The influence of 2,4,6-tribromophenyl isocyanate on the cellular and nuclear uptake of the SV 40 T antigen nuclear localization sequence.

Abstract

The objective of the present study was to evaluate the influence of 2,4,6-tribromophenyl isocyanate (TBPI) on the cellular and nuclear uptake of the fluorescein isothiocyanate (FITC) labeled SV 40 T antigen nuclear localization sequence in human LN18 and U373 glioma cells. Therefore, the FITC-labeled nuclear localization sequence (NLS) of the SV 40 T antigen was coupled to 2,4,6-TBPI. This TBPI-NLS conjugate was taken up by the cell nuclei of more than 90% of human malignant glioma cells. The nuclearly stained cells showed clear signs of cell death. However only up to 10% of the cells were stained after incubation with the TBPI-lacking NLS of the SV 40 T antigen together with free, unbound TBPI. These cells stayed alive. TBPI, when bound to small peptides, may be an important component for future drugs against gliomas.

• Authors: Stefan Heckl, Alexander Sturzu, Alireza Gharabaghi, Hartmut Echner, Thomas Nagele
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 583 Issue 1 Pg. 32-6 (Mar 31 2008) ISSN: 0014-2999 [Print] Netherlands
• PMID: 18262518 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 2,4,6-tribromophenyl isocyanate
• Antigens, Polyomavirus Transforming
• Fluorescent Dyes
• Isocyanates
• Fluorescein-5-isothiocyanate

Topics

• Antigens, Polyomavirus Transforming (metabolism)
• Biological Transport, Active
• Brain Neoplasms (metabolism)
• Cell Line, Tumor
• Cell Nucleus (metabolism, ultrastructure)
• Flow Cytometry
• Fluorescein-5-isothiocyanate
• Fluorescent Dyes
• Glioma (metabolism)
• Humans
• Isocyanates (pharmacology)
• Microscopy, Confocal
• Spectrometry, Mass, Electrospray Ionization