Human in vivo cellular response to a cross-linked acellular collagen implant.

Abstract

BACKGROUND:

Hernia surgery, in particular parastomal hernia mesh repair and new techniques for hernia prevention, require novel biomaterials that avoid fibrosis and potential bowel erosion, while retaining adequate strength for their intended purpose. The aim was to evaluate the human host response to an acellular porcine-derived cross-linked collagen implant.

METHODS:

In a prospective pilot study on prevention of parastomal herniation, 15 patients undergoing loop stoma formation had an implant placed within the anterior abdominal wall. Histopathology and immunohistochemistry were performed to analyse the implant qualitatively and, where appropriate, quantitatively for biocompatibility, degradation, cellular infiltration, neo-extracellular matrix (ECM) formation and neovascularization.

RESULTS:

At a median of 7 (range 1-8) months, 12 of 15 patients had stoma reversal and 11 implant biopsies were obtained. In biopsies from ten of the 11 patients all responses were limited to the periphery of the implant and native pores. There was a minimal inflammatory response and minimal degradation of the implant. Fibroblastic and neovascular infiltration were noted, as was matrix metalloproteinase 1 activity with organized deposition of host collagen, fibronectin and laminin.

CONCLUSION:

The collagen implant demonstrated excellent biocompatibility and resistance to degradation in most patients. However, fibrovascular in-growth and ECM deposition were limited. This implant has excellent potential for soft tissue reinforcement.

• Authors: T M Hammond, J Chin-Aleong, H Navsaria, N S Williams
• Journal: The British journal of surgery (Br J Surg) Vol. 95 Issue 4 Pg. 438-46 (Apr 2008) ISSN: 1365-2168 [Electronic] England
• PMID: 18256994 (Publication Type: Evaluation Study, Journal Article)
• Copyright: 2008 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.

Chemical References

• Permacol
• Collagen

Topics

• Biopsy
• Collagen (therapeutic use)
• Hernia (prevention & control)
• Host vs Graft Reaction
• Humans
• Ileal Diseases (prevention & control)
• Ileostomy (methods)
• Ileum (pathology)
• Immunohistochemistry
• Pilot Projects
• Prospective Studies
• Prosthesis Implantation (methods)
• Reoperation (statistics & numerical data)
• Surgical Stomas (pathology)
• Tensile Strength