Replicating adenovirus HIV/SIV recombinant priming alone or in combination with a gp140 protein boost results in significant control of viremia following a SHIV89.6P challenge in Mamu-A*01 negative rhesus macaques.

Abstract

Previously, replicating adenovirus type 5 host range (Ad5hr)-HIV/SIV recombinant priming in combination with SIV envelope boosting, resulted in significant, durable protection in 39% of rhesus macaques after SIVmac251 challenge. Both Env-specific antibody mediating ADCC, and cellular immunity correlated with protection. Here we evaluate the relative immunogenicities of novel HIV proteins and their contribution to protection in a SHIV89.6P model. All groups were primed with Ad-HIVenv89.6P, SIVgag239, and SIVnef239 recombinants. One group was not boosted, one received HIV89.6Pgp140DeltaCFI protein, and one a novel HIV-1 poly-peptide "peptomer". The HIV89.6Pgp140DeltaCFI protein in adjuvant strongly boosted Env-specific antibody and memory T cell responses in blood and tissue, resulting in significant reductions in acute and set point viremia. Macaques not boosted, showed a significant reduction in set point viremia, a full 32 weeks after the last Ad priming immunization. The HIV peptomer-boosted group showed a trend toward chronic viremia reduction, but was not protected.

• Authors: L Jean Patterson, Jennifer Beal, Thorsten Demberg, Ruth H Florese, Nina Malkevich, David Venzon, Kris Aldrich, Ersell Richardson, V S Kalyanaraman, Irene Kalisz, Eun Mi Lee, David C Montefiori, Frank A Robey, Marjorie Robert-Guroff
• Journal: Virology (Virology) Vol. 374 Issue 2 Pg. 322-37 (May 10 2008) ISSN: 0042-6822 [Print] United States
• PMID: 18252262 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• AIDS Vaccines
• HIV Antibodies
• Histocompatibility Antigens Class I
• Mamu-A 01 antigen
• SAIDS Vaccines
• env Gene Products, Human Immunodeficiency Virus
• gp140 envelope protein, Human immunodeficiency virus 1

Topics

• AIDS Vaccines (administration & dosage, immunology)
• Adenoviridae (genetics, physiology)
• Animals
• Genetic Vectors
• HIV Antibodies (blood)
• HIV Infections (prevention & control)
• HIV-1 (genetics, immunology, pathogenicity)
• Histocompatibility Antigens Class I (metabolism)
• Humans
• Immunization
• Immunization, Secondary
• Macaca mulatta
• Male
• Recombination, Genetic
• SAIDS Vaccines (administration & dosage, immunology)
• Simian Acquired Immunodeficiency Syndrome (prevention & control)
• Simian Immunodeficiency Virus (genetics, immunology, pathogenicity, radiation effects)
• T-Lymphocytes (immunology)
• Viremia (prevention & control)
• Virus Replication
• env Gene Products, Human Immunodeficiency Virus (administration & dosage, immunology)