The pap1(+) gene of fission yeast is transcriptionally regulated by nitrosative and nutritional stress.

Abstract

In the current work, regulation of the pap1(+) gene was investigated by the use of the pap1(+)-lacZ fusion gene and semi-quantitative reverse transcriptase-PCR. The synthesis of beta-galactosidase from the pap1(+)-lacZ fusion gene was significantly enhanced by nitric oxide (NO)-generating sodium nitroprusside (SNP) and nitrogen starvation. However, the induction by SNP and nitrogen starvation was observed to be much less in the Pap1p-negative cells harboring the fusion gene. Exogenous NO was more effectively scavenged in the Pap1p-positive cells than in the Pap1p-negative cells. Oxidative stress such as superoxide anion, hydrogen peroxide and cadmium could not give rise to an effect on the synthesis of beta-galactosidase from the fusion gene. The pap1(+) mRNA level was elevated in the wild-type cells by SNP and nitrogen starvation. Catalase activity, a major enzyme positively regulated by Pap1p, was significantly increased only in the Pap1p-positive cells by SNP. In brief, it is demonstrated that transcription of the Schizosaccharomyces pombe pap1(+) gene is positively regulated by nitrosative and nutritional stress in a Pap1p-dependent manner.

• Authors: Hyeon-Jung Kim, Ha-Yun Jung, Chang-Jin Lim
• Journal: FEMS microbiology letters (FEMS Microbiol Lett) Vol. 280 Issue 2 Pg. 176-81 (Mar 2008) ISSN: 0378-1097 [Print] England
• PMID: 18248428 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Basic-Leucine Zipper Transcription Factors
• Nitrates
• Pancreatitis-Associated Proteins
• Pap1 protein, S pombe
• REG3A protein, human
• Schizosaccharomyces pombe Proteins
• Nitrogen

Topics

• Basic-Leucine Zipper Transcription Factors (genetics, metabolism)
• Gene Expression Regulation, Fungal
• Nitrates (metabolism)
• Nitrogen (metabolism)
• Nitrosation
• Nutrition Assessment
• Pancreatitis-Associated Proteins
• Schizosaccharomyces (genetics, physiology)
• Schizosaccharomyces pombe Proteins (genetics, metabolism)