[Inhibition of bFGF gene expression and tumor angiogenesis of orthotopic implantation of human gastric carcinoma by N-desulfated heparin].

Abstract

OBJECTIVE:

To investigate the effect of N-desulfated heparin on tumor metastasis, tumor angiogenesis and basic fibroblast growth factor(bFGF) gene expression of orthotopically implanted human gastric carcinoma in NOD-SCID mice.

METHODS:

Human gastric cancer SGC-7901 tissues were orthotopically implanted into the stomach of the NOD-SCID mice. Twenty mice were randomly divided into two groups which received either intravenous injection of 0.9% NaCl solution(0.9%NaCl solution group) or 10 mg/kg N-desulfated heparin (N-desulfated heparin group) twice a week for three weeks. Mice were sacrificed six weeks after tumor implantation. Tissues from stomach and other organs were obtained for histopathological evaluation. The intratumoral microvessel density (MVD) in tumor was evaluated immunohistochemically. Real time PCR was used to detect bFGF mRNA expression.

RESULTS:

The tumor metastasis rates were 9/10 in 0.9% NaCl solution group and 2/10 in N-desulfated heparin group(P<0.05).MVD was 9.1+/-3.4 in 0.9% NaCl solution group and 4.7+/-1.8 in N-desulfated heparin group (t=3.617,P<0.05). bFGF mRNA expression was lower in N-desulfated heparin group(2.60+/-0.56%)than that in 0.9% NaCl solution group(30.65+/-6.84%).

CONCLUSION:

N-desulfated heparin can inhibit the metastasis of gastric cancer through inhibiting tumor bFGF gene expression and tumor angiogenesis with no obvious anticoagulant activity.

• Authors: Ming-xiang Chen, Jin-lian Chen, Jin-lai Lu, Jing Hong, Wei-xiong Chen, Jin-shui Zhu, Ni-wei Chen, Jian-guo Geng
• Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (Zhonghua Yi Xue Yi Chuan Xue Za Zhi) Vol. 25 Issue 1 Pg. 78-81 (Feb 2008) ISSN: 1003-9406 [Print] China
• PMID: 18247311 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• heparin, N-desulfated
• Fibroblast Growth Factor 2
• Heparin

Topics

• Animals
• Fibroblast Growth Factor 2 (genetics)
• Gene Expression Regulation, Neoplastic (drug effects, genetics)
• Heparin (analogs & derivatives, pharmacology, therapeutic use)
• Humans
• Male
• Mice
• Mice, Inbred NOD
• Mice, SCID
• Neoplasm Metastasis
• Neoplasm Transplantation
• Neovascularization, Pathologic (drug therapy)
• Polymerase Chain Reaction
• RNA, Messenger (genetics, metabolism)
• Stomach Neoplasms (blood supply, drug therapy, genetics)