A molecular signature of gastric metaplasia arising in response to acute parietal cell loss.
Abstract | BACKGROUND & AIMS: METHODS: Emerging SPEM lineages in gastrin-deficient mice treated with DMP-777 were examined for immunolocalization of TFF2, intrinsic factor, and Mist1, and morphologically with electron microscopy. Emerging SPEM was isolated with laser-capture microdissection and RNA was analyzed using gene microarrays. Immunohistochemistry in mouse and human samples was used to confirm up-regulated transcripts. RESULTS: DMP-777-induced SPEM was immunoreactive for TFF2 and the differentiated chief cell markers, Mist1 and intrinsic factor, suggesting that SPEM derived from transdifferentiation of chief cells. Microarray analysis of microdissected SPEM lineages induced by DMP-777 showed up-regulation of transcripts associated with G1/S cell-cycle transition including minichromosome maintenance deficient proteins, as well as a number of secreted factors, including human epididymis 4 (HE4). HE4, which was absent in the normal stomach, was expressed in SPEM of human and mouse and in intestinal metaplasia and gastric cancer in human beings. CONCLUSIONS: Although traditionally metaplasia was thought to originate from normal mucosal progenitor cells, these studies indicate that SPEM evolves through either transdifferentiation of chief cells or activation of a basal cryptic progenitor. In addition, induction of metaplasia elicits the expression of secreted factors, such as HE4, relevant to gastric preneoplasia.
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Authors | Koji Nozaki, Masako Ogawa, Janice A Williams, Bonnie J Lafleur, Vivian Ng, Ronny I Drapkin, Jason C Mills, Stephen F Konieczny, Sachiyo Nomura, James R Goldenring |
Journal | Gastroenterology
(Gastroenterology)
Vol. 134
Issue 2
Pg. 511-22
(Feb 2008)
ISSN: 1528-0012 [Electronic] United States |
PMID | 18242217
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Basic Helix-Loop-Helix Transcription Factors
- Bhlha15 protein, mouse
- Carrier Proteins
- Cell Cycle Proteins
- DEFB126 protein, human
- DNA-Binding Proteins
- Epididymal Secretory Proteins
- Fetal Proteins
- Gastrins
- Intercellular Signaling Peptides and Proteins
- Mcm3 protein, mouse
- Microtubule-Associated Proteins
- Mucins
- Muscle Proteins
- Nuclear Proteins
- Peptides
- TACC3 protein, mouse
- TFF2 protein, human
- TFF2 protein, mouse
- Trefoil Factor-2
- beta-Defensins
- spasmolytic polypeptide
- Intrinsic Factor
- Minichromosome Maintenance Complex Component 3
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Topics |
- Animals
- Atrophy
- Basic Helix-Loop-Helix Transcription Factors
(metabolism)
- Carrier Proteins
(metabolism)
- Cell Cycle Proteins
(metabolism)
- Cell Transformation, Neoplastic
(metabolism, pathology)
- Chief Cells, Gastric
(metabolism, pathology)
- DNA-Binding Proteins
(metabolism)
- Epididymal Secretory Proteins
(metabolism)
- Fetal Proteins
(metabolism)
- Gastric Fundus
(metabolism, pathology)
- Gastric Mucosa
(metabolism, pathology)
- Gastrins
(metabolism)
- Humans
- Intercellular Signaling Peptides and Proteins
- Intrinsic Factor
(metabolism)
- Metaplasia
(metabolism, pathology)
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Microtubule-Associated Proteins
- Minichromosome Maintenance Complex Component 3
- Mucins
(metabolism)
- Muscle Proteins
(metabolism)
- Nuclear Proteins
(metabolism)
- Parietal Cells, Gastric
(metabolism, pathology)
- Peptides
(metabolism)
- Precancerous Conditions
(metabolism, pathology)
- Stomach Neoplasms
(metabolism, pathology, physiopathology)
- Trefoil Factor-2
- beta-Defensins
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