Abstract | AIM: METHODS: The model of insulin resistance in 3T3-L1 adipocytes was established by adding palmic acid (0.5 mmol/L) to the culture medium. Berberine treatment was performed at the same time. Glucose uptake rate was determined by the 2-deoxy-[(3)H]- D-glucose method. The levels of IkB kinase beta (IKKbeta) Ser(181) phosphorylation, insulin receptor substrate-1(IRS-1) Ser(307) phosphorylation, expression of IKKbeta, IRS-1, nuclear transcription factor kappaB p65 (NF-kappaB p65), phosphatidylinositol-3-kinase p85 (PI-3K p85) and glucose transporter 4 (GLUT4) proteins were detected by Western blotting. The distribution of NF-kappaB p65 proteins inside the adipocytes was observed through confocal laser scanning microscopy (CLSM). RESULTS: After the intervention of palmic acid for 24 h, the insulin-stimulated glucose transport in 3T3-L1 adipocytes was inhibited by 67%. Meanwhile, the expression of IRS-1 and PI-3K p85 protein was reduced, while the levels of IKKbeta Ser(181) and IRS-1 Ser(307) phosphorylation, and nuclear translocation of NF-kappaB p65 protein were increased. However, the above indexes, which indicated the existence of insulin resistance, were reversed by berberine although the expression of GLUT4, IKKbeta and total NF-kappaB p65 protein were not changed during this study. CONCLUSION:
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Authors | Ping Yi, Fu-Er Lu, Li-Jun Xu, Guang Chen, Hui Dong, Kai-Fu Wang |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 14
Issue 6
Pg. 876-83
(Feb 14 2008)
ISSN: 1007-9327 [Print] United States |
PMID | 18240344
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adaptor Proteins, Signal Transducing
- Fatty Acids, Nonesterified
- Insulin
- Insulin Receptor Substrate Proteins
- Irs1 protein, mouse
- Transcription Factor RelA
- Berberine
- Serine
- Phosphatidylinositol 3-Kinases
- I-kappa B Kinase
- Ikbkb protein, mouse
- Glucose
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Topics |
- 3T3-L1 Cells
- Active Transport, Cell Nucleus
(physiology)
- Adaptor Proteins, Signal Transducing
(metabolism)
- Adipocytes
(cytology, metabolism)
- Animals
- Berberine
(metabolism)
- Fatty Acids, Nonesterified
(metabolism)
- Glucose
(metabolism)
- I-kappa B Kinase
(metabolism)
- Insulin
(metabolism)
- Insulin Receptor Substrate Proteins
- Insulin Resistance
(physiology)
- Mice
- Phosphatidylinositol 3-Kinases
(metabolism)
- Serine
(metabolism)
- Transcription Factor RelA
(metabolism)
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