HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Cytotoxic effects and pro-apoptotic mechanism of TBIDOM, a novel dehydroabietylamine derivative, on human hepatocellular carcinoma SMMC-7721 cells.

Abstract
We have investigated the antiproliferative effects of TBIDOM (N-(4-(2,2,2-trifluoroethyl) benzylidene) (7-isopropyl-1,4a-dimethyl-1,2,3,4,4a,9,10,10a-octahydrophenanthren-1-yl) meth-anamine) and have explored its possible mechanisms on human hepatocellular carcinoma SMMC-7721 cells. The proliferative status of cells treated with TBIDOM was measured by the colorimetric MTT assay. Cellular apoptosis was analysed using Hoechst 33342 staining and flow cytometry. Reduction of mitochondrial membrane potential (Delta psi(m)) was also detected by flow cytometry. Western blotting assay was used to evaluate the release of cytochrome c and expression of p53, Bcl-2 and Bax proteins. It was shown that TBIDOM displayed a significant inhibitory effect on growth of SMMC-7721 cells in a dose- and time-dependent manner. Hoechst 33342 staining and flow cytometry analysis showed an increase of apoptosis rate and decrease of mitochondrial membrane potential after SMMC-7721 cells were exposed to TBIDOM for 24 h. Pretreatment of SMMC-7721 cells with TBIDOM significantly induced a decrease of Bcl-2 protein expression and an increase of caspase-3 activity and Bax protein expression. The results indicated that TBIDOM could effectively inhibit proliferation by induction of apoptosis and could be a promising candidate in the development of a novel class of antitumour agent.
AuthorsFu Li, Ling He, Zhan-Qian Song, Jin-Cheng Yao, Xiao-Ping Rao, Hai-Tao Li
JournalThe Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 60 Issue 2 Pg. 205-11 (Feb 2008) ISSN: 0022-3573 [Print] England
PMID18237468 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • (N-(4-(2,2,2-trifluoroethyl) benzylidene) (7-isopropyl-1,4a-dimethyl-1,2,3,4,4a,9,10,10a-octahydrophenanthren-1-yl) meth-anamine)
  • Abietanes
  • Antineoplastic Agents
  • Benzyl Compounds
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-2-Associated X Protein
  • Caspase 3
Topics
  • Abietanes (administration & dosage, pharmacology)
  • Antineoplastic Agents (administration & dosage, pharmacology)
  • Apoptosis
  • Benzyl Compounds (administration & dosage, pharmacology)
  • Blotting, Western
  • Carcinoma, Hepatocellular (drug therapy)
  • Caspase 3 (drug effects, metabolism)
  • Cell Line, Tumor
  • Cell Proliferation (drug effects)
  • Colorimetry
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • Gene Expression Regulation (drug effects)
  • Humans
  • Liver Neoplasms (drug therapy)
  • Membrane Potential, Mitochondrial (drug effects)
  • Proto-Oncogene Proteins c-bcl-2 (drug effects, metabolism)
  • Time Factors
  • bcl-2-Associated X Protein (drug effects, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: