Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations.

Abstract

PURPOSE:

To study the ganglion cells (GC) in the terminal bowel of rats with ethylenethiourea (ETU) induced anorectal malformations (ARM).

METHODS:

The animals were divided into three groups: Group A--normal fetuses from pregnant rats that were not administered ETU; Group B--fetuses without ARM born from pregnant rats that were administered ETU and Group C--fetuses with ARM born from pregnant rats that received ETU. ETU was administered on the 11th day of pregnancy at the dose of 125 mg/kg body weight by gastric gavage. The rats had cesarean section on the 21st day of gestation. The fetuses terminal bowel tissue was analyzed by immunohistochemistry to demonstrate ganglion cells.

RESULTS:

Statistically significant differences were found between groups A, B and C regarding ganglion cell densities. Group A had the highest cell density, followed by Group B and the lowest density was found in Group C.

CONCLUSION:

Ganglion cell densities are decreased in the terminal bowel of rats with ARM.

• Authors: Maurício Macedo, José Luiz Martins, Karine Furtado Meyer, Iberê Cauduro Soares
• Journal: Acta cirurgica brasileira (Acta Cir Bras) 2007 Nov-Dec Vol. 22 Issue 6 Pg. 441-5 ISSN: 0102-8650 [Print] Brazil
• PMID: 18235931 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• S100 Proteins
• Ethylenethiourea

Topics

• Anal Canal (abnormalities, innervation, pathology)
• Animals
• Cell Count
• Disease Models, Animal
• Enteric Nervous System (drug effects, pathology)
• Ethylenethiourea
• Female
• Ganglia (cytology)
• Immunohistochemistry
• Pregnancy
• Rats
• Rats, Wistar
• Rectum (abnormalities, innervation, pathology)
• S100 Proteins (analysis)