Abstract | UNLABELLED: METHODS: Four- to 5-week-old BALB/c mice of either sex were infected intravenously with lethal dose of 3 x 10(5) pfu of Japanese encephalitis virus (JEV). By the 9th day post- infection, all untreated animals succumbed to the infection. Arctigenin was dissolved in DMSO at a concentration of 0.5 mg/mL and stored at 4 degrees C. After one day following virus inoculation, animals were given arctigenin intraperitoneally, twice daily (10 mg/kg of body weight) for next 7 days. RESULTS: CONCLUSIONS: In conclusion, our findings provide a novel mechanistic insight into the actions of arctigenin in JE. Results from our in vivo and in vitro experiments clearly indicate that arctigenin reduced (i) viral load and viral replication within the brain, (ii) neuronal death and (iii) secondary inflammation and oxidative stress resulting from microglial activation, thereby suggesting its potential for treating JE. The antiviral, neuroprotective, anti-inflammatory and antioxidative effects of arctigenin successfully reduced the severity of disease induced by JEV.
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Authors | Vivek Swarup, Joydeep Ghosh, Manoj Kumar Mishra, Anirban Basu |
Journal | The Journal of antimicrobial chemotherapy
(J Antimicrob Chemother)
Vol. 61
Issue 3
Pg. 679-88
(Mar 2008)
ISSN: 1460-2091 [Electronic] England |
PMID | 18230688
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Furans
- Japanese Encephalitis Vaccines
- Lignans
- arctigenin
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Topics |
- Animals
- Cell Death
(drug effects, physiology)
- Encephalitis, Japanese
(drug therapy, prevention & control, virology)
- Female
- Furans
(pharmacology, therapeutic use)
- Japanese Encephalitis Vaccines
(pharmacology, therapeutic use)
- Lignans
(pharmacology, therapeutic use)
- Male
- Mice
- Mice, Inbred BALB C
- Phytotherapy
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