Preparation of basement membrane components from EHS tumors.

Abstract

This unit describes methods for passaging and harvesting the basement membrane matrix-producing EHS tumor and for the subsequent isolation of a crude mixture of basement membrane components termed Matrigel, which promotes the differentiation of a variety of epithelial, endothelial, and neuronal cells. Procedures for the isolation of the adhesive glycoprotein laminin-1 and of type IV collagen are also included. Support protocols cover the maintenance and harvesting of EHS tumors in mice and maintenance of mice on a lathrogenic diet.

• Authors: H K Kleinman
• Journal: Current protocols in cell biology (Curr Protoc Cell Biol) Vol. Chapter 10 Pg. Unit 10.2 (May 2001) ISSN: 1934-2616 [Electronic] United States
• PMID: 18228296 (Publication Type: Journal Article)

Chemical References

• Biocompatible Materials
• Collagen Type IV
• Drug Combinations
• Laminin
• Proteoglycans
• laminin 1
• matrigel
• Collagen

Topics

• Animals
• Basement Membrane (metabolism)
• Biocompatible Materials (isolation & purification, metabolism)
• Collagen (isolation & purification, metabolism)
• Collagen Type IV (isolation & purification, metabolism)
• Cytological Techniques
• Drug Combinations
• Laminin (isolation & purification, metabolism)
• Mice
• Neoplasms, Experimental (metabolism, pathology)
• Proteoglycans (isolation & purification, metabolism)
• Tumor Cells, Cultured