[Expression of HSP27 in colorectal carcinoma and its relationship with lymphatic metastasis].

Abstract

OBJECTIVE:

To investigate the relationship between heat shock protein 27 (HSP27) expression and lymphatic metastasis of colorectal carcinoma (CRC).

METHODS:

Immunohistochemistry was used to detect HSP27 expression in 68 specimens of human CRC. The expression of HSP27 mRNA and protein was also detected in two colorectal carcinoma cell lines with different lymphatic metastasis potentials by RT-PCR, Western blotting and immunohistochemistry.

RESULTS:

HSP27 expression was not related to such clinicopathological factors as the patient's age, gender, histological grade, lymphatic metastasis or clinical stages (P>0.05), but overexpression of HSP27 showed significant inverse correlation to lymphatic metastasis of CRC (P=0.035). HSP27 mRNA and protein were expressed at low levels in SW620 cells with high potentials for lymphatic metastasis, and at high levels in SW480 cells that had low lymphatic metastasis potentials.

CONCLUSION:

HSP27 expression can be inversely correlated to metastatic behavior of CRC cells, and may play a role in suppressing the metastasis of CRC.

• Authors: Liang Zhao, Zu-Guo Li, Yan-Qing Ding
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 28 Issue 1 Pg. 41-4 (Jan 2008) ISSN: 1673-4254 [Print] China
• PMID: 18227023 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HSP27 Heat-Shock Proteins
• RNA, Messenger

Topics

• Adenocarcinoma (genetics, metabolism, pathology)
• Adult
• Aged
• Aged, 80 and over
• Blotting, Western
• Cell Line, Tumor
• Colorectal Neoplasms (genetics, metabolism, pathology)
• Female
• Gene Expression Regulation, Neoplastic
• HSP27 Heat-Shock Proteins (biosynthesis, genetics)
• Humans
• Immunohistochemistry
• Lymphatic Metastasis
• Male
• Middle Aged
• RNA, Messenger (biosynthesis, genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Young Adult