Abstract | BACKGROUND: MATERIALS AND METHODS:
Sabarubicin-induced mtDNA cleavage was detected by Southern blotting and mitochondrial mRNA expression was analyzed by real-time PCR. Apoptosis was studied in mtDNA-depleted (theta0) and parental (theta+) A2780 cells detecting nuclear DNA fragmentation using ELISA and cytofluorimetrically using Annexin V/PI staining. Mitochondrial membrane potential was studied using the cyanine dye JC-1. RESULTS:
Sabarubicin induced mtDNA cleavage in the A2780 cells, but this damage did not affect mitochondrial mRNA expression. Apoptosis was induced by sabarubicin in theta0 as well as in theta+ cells. CONCLUSION: The results showed that mtDNA did not influence anthracycline-induced apoptosis in A2780 cells.
|
Authors | Alessandro Bressan, Federica Nardelli, Daniela Bellarosa, Mario Bigioni, Giuseppe Curcurù, Barbara Curatella, Attilio Crea, Carlo Alberto Maggi, Stefano Manzini, Monica Binaschi |
Journal | Anticancer research
(Anticancer Res)
2007 Nov-Dec
Vol. 27
Issue 6B
Pg. 4039-46
ISSN: 0250-7005 [Print] Greece |
PMID | 18225568
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antibiotics, Antineoplastic
- DNA, Mitochondrial
- Disaccharides
- Doxorubicin
- sabarubicin
|
Topics |
- Antibiotics, Antineoplastic
(pharmacokinetics, pharmacology)
- Apoptosis
(drug effects, genetics)
- Blotting, Southern
- Cell Line, Tumor
- DNA, Mitochondrial
(metabolism)
- Disaccharides
(pharmacokinetics, pharmacology)
- Doxorubicin
(analogs & derivatives, pharmacokinetics, pharmacology)
- Female
- Gene Expression
(drug effects)
- Humans
- Membrane Potential, Mitochondrial
(drug effects)
- Mitochondria
(drug effects, metabolism)
- Ovarian Neoplasms
(drug therapy, genetics, pathology)
- Reverse Transcriptase Polymerase Chain Reaction
|