Abstract | PURPOSE: EXPERIMENTAL DESIGN: The effect of flavopiridol on rhabdoid cells was tested in vitro using survival, cell cycle, and apoptosis assays. Its effect was assessed in vivo using xenografted rhabdoid tumor models. Immunoblot and immunohistochemical analysis was used to assess the effect of flavopiridol on cyclin D1 and p21 expression in vitro and in vivo, respectively. RESULTS: Nanomolar concentrations of flavopiridol inhibited rhabdoid cell growth (IC(50) approximately 200 nmol/L), induced G(1) and G(2) arrest, and apoptosis in vitro in a concentration-dependent manner. These effects were correlated with the down-modulation of cyclin D1, up-regulation of p21, and induction of caspase 3/7 activities. Flavopiridol (at 7.5 mg/kg) significantly inhibited the growth of xenografted rhabdoid tumors, and its effect was correlated with the induction of p21 and down-modulation of cyclin D1. CONCLUSIONS:
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Authors | Melissa E Smith, Velasco Cimica, Srinivasa Chinni, Kavitha Challagulla, Sridhar Mani, Ganjam V Kalpana |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 14
Issue 2
Pg. 523-32
(Jan 15 2008)
ISSN: 1078-0432 [Print] United States |
PMID | 18223228
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Cyclin-Dependent Kinase Inhibitor p21
- Flavonoids
- Piperidines
- Protein Kinase Inhibitors
- Cyclin D1
- alvocidib
- Cyclin-Dependent Kinases
- Caspases
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Caspases
(metabolism)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cyclin D1
(metabolism)
- Cyclin-Dependent Kinase Inhibitor p21
(metabolism)
- Cyclin-Dependent Kinases
(metabolism)
- Drug Screening Assays, Antitumor
- Flavonoids
(pharmacology)
- Humans
- Mice
- Mice, SCID
- Neoplasm Transplantation
- Piperidines
(pharmacology)
- Protein Kinase Inhibitors
(pharmacology)
- Rhabdoid Tumor
(drug therapy, enzymology, metabolism, pathology)
- Xenograft Model Antitumor Assays
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