Abstract |
Ischemia-reperfusion (IR) causes human lung injury in association with the release of atrial and brain natriuretic peptides ( ANP and BNP), but the role of ANP/BNP in IR lung injury is unknown. ANP and BNP bind to natriuretic peptide receptor-A (NPR-A) generating cGMP and to NPR-C, a clearance receptor that can decrease intracellular cAMP. To determine the role of NPR-A signaling in IR lung injury, we administered the NPR-A blocker anantin in an in vivo SWR mouse preparation of unilateral lung IR. With uninterrupted ventilation, the left pulmonary artery was occluded for 30 min and then reperfused for 60 or 150 min. Anantin administration decreased IR-induced Evans blue dye extravasation and wet weight in the reperfused left lung, suggesting an injurious role for NPR-A signaling in lung IR. In isolated mouse lungs, exogenous ANP (2.5 nM) added to the perfusate significantly increased the filtration coefficient sevenfold only if lungs were subjected to IR. This effect of ANP was also blocked by anantin. Unilateral in vivo IR increased endogenous plasma ANP, lung cGMP concentration, and lung protein kinase G (PKG(I)) activation. Anantin enhanced plasma ANP concentrations and attenuated the increase in cGMP and PKG(I) activation but had no effect on lung cAMP. These data suggest that lung IR triggered ANP release and altered endothelial signaling so that NPR-A activation caused increased pulmonary endothelial permeability.
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Authors | Jeffrey M Dodd-o, Maria L Hristopoulos, Kathleen Kibler, Jolanta Gutkowska, Suhayla Mukaddam-Daher, Alfredo Gonzalez, Laura E Welsh-Servinsky, David B Pearse |
Journal | American journal of physiology. Lung cellular and molecular physiology
(Am J Physiol Lung Cell Mol Physiol)
Vol. 294
Issue 4
Pg. L714-23
(Apr 2008)
ISSN: 1040-0605 [Print] United States |
PMID | 18223163
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cell Adhesion Molecules
- Microfilament Proteins
- Phosphoproteins
- vasodilator-stimulated phosphoprotein
- Atrial Natriuretic Factor
- Cyclic AMP
- Guanylate Cyclase
- Receptors, Atrial Natriuretic Factor
- atrial natriuretic factor receptor A
- Cyclic GMP
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Topics |
- Animals
- Atrial Natriuretic Factor
(metabolism, pharmacology)
- Cell Adhesion Molecules
(drug effects, physiology)
- Cyclic AMP
(metabolism)
- Cyclic GMP
(metabolism)
- Guanylate Cyclase
(physiology)
- Lung
(drug effects, physiology, physiopathology)
- Male
- Mice
- Mice, Inbred Strains
- Microfilament Proteins
(drug effects, physiology)
- Perfusion
- Phosphoproteins
(drug effects, physiology)
- Receptors, Atrial Natriuretic Factor
(physiology)
- Reperfusion Injury
(physiopathology)
- Respiratory Distress Syndrome
(physiopathology)
- Signal Transduction
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