Evaluation of recombinant modified vaccinia Ankara virus-based rhesus cytomegalovirus vaccines in rhesus macaques.

Abstract

A vaccine consisting of rhesus cytomegalovirus (RhCMV) pp65-2, gB and IE1 expressed via modified vaccinia Ankara (MVA) was evaluated in rhesus macaques with or without prior priming with expression plasmids for the same antigens. Following two MVA treatments, comparable levels of anti-gB, pp65-2 and neutralizing antibody responses, and pp65-2- and IE1-specific cellular immune responses were detected in both vaccinated groups. Similar reductions in plasma peak viral loads were observed in these groups compared to untreated controls. This study demonstrates the immunogenicity and protective efficacy of rMVA-based RhCMV subunit vaccines in a primate host and warrants further investigation to improve the efficacy of subunit vaccines against CMV.

• Authors: Yujuan Yue, Zhongde Wang, Kristina Abel, Jinliang Li, Lisa Strelow, Angelo Mandarino, Meghan K Eberhardt, Kimberli A Schmidt, Don J Diamond, Peter A Barry
• Journal: Medical microbiology and immunology (Med Microbiol Immunol) Vol. 197 Issue 2 Pg. 117-23 (Jun 2008) ISSN: 0300-8584 [Print] Germany
• PMID: 18196272 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Antibodies, Viral
• Cytomegalovirus Vaccines
• Vaccines, DNA
• Vaccines, Subunit
• Viral Proteins

Topics

• Animals
• Antibodies, Viral (blood)
• Cytomegalovirus (genetics, immunology)
• Cytomegalovirus Vaccines (genetics, immunology)
• Immunization, Secondary
• Macaca mulatta
• Neutralization Tests
• T-Lymphocytes (immunology)
• Vaccines, DNA
• Vaccines, Subunit (genetics, immunology)
• Vaccinia virus (genetics)
• Viral Load
• Viral Proteins (genetics, immunology)