Influence of different extraction parameters on a solid-phase dynamic extraction for the gas chromatographic determination of d-limonene degradation products by using a fractional factorial design.

Through the application of a solid-phase dynamic extraction (SPDE) method for the determination of volatile substances, different parameters may influence the extraction and desorption process and are, therefore, vital for the determination of the extracted substances via gas chromatography (GC). In this study, the influence of extraction und desorption parameters of an SPDE-GC method for the determination of the d-limonene degradation products, namely, alpha-terpineol, (-)-carveol, and (S)-carvone, in an aqueous model system was evaluated using a factorial fractional design. The aim was to reduce the number of factors that should be considered for the optimization of an SPDE procedure for different applications. It could be shown that the extraction efficiency of alpha-terpineol, (-)-carveol, and (S)-carvone is significantly influenced by the extraction parameters incubation temperature, number of extraction strokes, and amount of added NaCl. All 3 parameters have a positive effect on the extraction and determination of the examined d-limonene degradation products. Due to the identification of significant factors on the basis of an experimental design, the results of this study can be very useful for further development of SPDE methods for different applications.
AuthorsMarion Kamphoff, Tobias Thiele, Benno Kunz
JournalJournal of AOAC International (J AOAC Int) 2007 Nov-Dec Vol. 90 Issue 6 Pg. 1623-7 ISSN: 1060-3271 [Print] United States
PMID18193739 (Publication Type: Journal Article)
Chemical References
  • Cyclohexenes
  • Indicators and Reagents
  • Monoterpenes
  • Terpenes
  • Sodium Chloride
  • carveol
  • carvone
  • limonene
  • Chromatography, Gas
  • Chromatography, High Pressure Liquid
  • Cyclohexenes (chemistry, isolation & purification)
  • Indicators and Reagents
  • Monoterpenes (analysis)
  • Sodium Chloride (analysis)
  • Temperature
  • Terpenes (chemistry, isolation & purification)

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