Abstract |
We attempted to assess the prophylactic effect of human umbilical cord blood-derived CD34(+) cells in experimental heatstroke. Anesthetized rats, 1 day before heat stress, were divided into 2 major groups and given CD34(-) cells (defined by 1 x 10(6) human cord blood lymphocytes and monocytes that contained <0.2% CD34(+) cells) or CD34(+) cells (defined by 1 x 10(6) human cord blood lymphocytes and monocytes that contained >95% CD34(+) cells). They were exposed to ambient temperature of 43 degrees C for 70 min to induce heatstroke. When the CD34(-) cells-treated or untreated rats underwent heat stress, their survival time values were found to be 20-24 min. Pretreatment with CD34(+) cells significantly increased survival time (123-351 min). As compared with normothermic controls, all CD34(-) cells-treated heatstroke animals displayed hypotension, hepatic and renal failure, hypercoagulable state, activated inflammation, and cerebral ischemia and injury. However, these heatstroke reactions all were significantly suppressed by CD34(+) cells pretreatment. In addition, the levels of interleukin-10 in plasma and glial cell line-derived neurotrophic factors in brain were all significantly increased after CD34(+) cell administration during heatstroke. Our data indicate that human umbilical cord-derived CD34(+) cells can be used as a prophylactic agent for experimental heatstroke.
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Authors | Wei-Shou Hwang, Sheng-Hsien Chen, Cheng-Hsien Lin, Hsiu-Kang Chang, Wei-Chun Chen, Mao-Tsun Lin |
Journal | Journal of pharmacological sciences
(J Pharmacol Sci)
Vol. 106
Issue 1
Pg. 46-55
(Jan 2008)
ISSN: 1347-8613 [Print] Japan |
PMID | 18187924
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, CD34
- Glial Cell Line-Derived Neurotrophic Factor
- Nitrates
- Nitrites
- Tumor Necrosis Factor-alpha
- Interleukin-10
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Topics |
- Animals
- Antigens, CD34
(analysis)
- Basal Ganglia
(metabolism)
- Cerebrovascular Disorders
(etiology, prevention & control)
- Cord Blood Stem Cell Transplantation
- Disease Models, Animal
- Fetal Blood
(cytology)
- Glial Cell Line-Derived Neurotrophic Factor
(metabolism)
- Heat Stroke
(complications, metabolism, prevention & control)
- Humans
- Interleukin-10
(blood)
- Kidney Diseases
(etiology, prevention & control)
- Liver Diseases
(etiology, prevention & control)
- Male
- Multiple Organ Failure
(etiology, metabolism, prevention & control)
- Nerve Degeneration
(etiology, prevention & control)
- Nitrates
(blood)
- Nitrites
(blood)
- Rats
- Rats, Sprague-Dawley
- Stem Cells
(immunology, metabolism)
- Time Factors
- Tumor Necrosis Factor-alpha
(blood)
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