HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Identification of a granulin-like transcript expressed during anoxic exposure and translated during aerobic recovery in a marine gastropod.

Abstract
A novel transcript encoding a cysteine-rich granulin-like peptide (l-grn) was identified in the hepatopancreas of the marine intertidal gastropod, Littorina littorea, an anoxia-tolerant species. Experimental exposure of snails to anoxia induced a gradual accumulation of l-grn transcripts over time, with expression regulated in vitro through elements responsive to second messengers of protein kinases A, C and G. Translation of this transcript was analyzed by examining l-grn association with ribosomes during normoxia, anoxia, and aerobic recovery. Transcripts of l-grn were associated with polysomes during normoxia, moved into the monosome fractions under anoxia, but shifted back to the polysomal fractions during aerobic recovery. Western blotting confirmed this with a granulin-like protein detected under normoxic conditions, but not during anoxia exposure. A significant increase in the precursor protein and peptide (L-GRN) was observed during the aerobic recovery period. The accumulation of l-grn transcripts during anoxic exposure and subsequent translation following the return to aerobic conditions may be a response to oxidant damage that occurs during re-oxygenation. Overall, the data show that the l-grn gene is anoxia-responsive in this species and may have pro-survival functions during the recovery period.
AuthorsKevin Larade, Kenneth B Storey
JournalGene (Gene) Vol. 410 Issue 1 Pg. 37-43 (Feb 29 2008) ISSN: 0378-1119 [Print] Netherlands
PMID18187271 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Intercellular Signaling Peptides and Proteins
  • Progranulins
  • RNA, Messenger
Topics
  • Amino Acid Sequence
  • Animals
  • Hypoxia (genetics)
  • Intercellular Signaling Peptides and Proteins (chemistry, genetics)
  • Marine Biology
  • Molecular Sequence Data
  • Mollusca (genetics)
  • Progranulins
  • RNA, Messenger (genetics)
  • Sequence Homology, Amino Acid
  • Transcription, Genetic

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: