Abstract | BACKGROUND: METHODS: RESULTS: Median muscular motilin binding was 3 and 8 fmol/g tissue in colon and ileum, respectively. In the gastroduodenal region the median was higher (93 fmol/g). In UC colonic muscular motilin binding was significantly increased compared to controls (7 vs. 3 fmol/g, P < or = 0.05). Expression in CD was similar to controls. Besides the binding found in the muscular compartment, motilin binding was also found in the mucosa, which was even higher than in the muscle (3 versus 11 and 8 versus 27 fmol/g for colon and ileum (P < or = 0.06), respectively). RT-PCR and immunohistochemistry confirmed the mucosal motilin receptor expression. The mucosal motilin receptors were located in the epithelial cells. In the muscular compartment receptors were strongly present in the myenteric plexus and weakly in the smooth muscle cells. In IBD tissue the expression pattern was not different. CONCLUSIONS: The motilin receptor is expressed in human colonic and ileal smooth muscle. Further, motilin receptor expression was also shown in the mucosa. Muscular binding in UC patients is increased but no different expression pattern was found.
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Authors | W Pascale Ter Beek, Eveline S M Muller, Marlies van den Berg, Martin J Meijer, Izäk Biemond, Cornelis B H W Lamers |
Journal | Inflammatory bowel diseases
(Inflamm Bowel Dis)
Vol. 14
Issue 5
Pg. 612-9
(May 2008)
ISSN: 1078-0998 [Print] England |
PMID | 18183601
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Messenger
- Receptors, Gastrointestinal Hormone
- Receptors, Neuropeptide
- motilin receptor
- Peroxidase
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Topics |
- Adolescent
- Adult
- Aged
- Autoradiography
- Colon
(metabolism, pathology)
- Female
- Gene Expression
(genetics)
- Humans
- Ileum
(metabolism, pathology)
- Immunohistochemistry
- Inflammatory Bowel Diseases
(genetics, metabolism, pathology)
- Intestinal Mucosa
(metabolism, pathology)
- Male
- Middle Aged
- Muscle, Smooth
(metabolism)
- Peroxidase
(metabolism)
- RNA, Messenger
(biosynthesis, genetics)
- Receptors, Gastrointestinal Hormone
(biosynthesis, genetics)
- Receptors, Neuropeptide
(biosynthesis, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
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