Absorption, biliary excretion, and metabolism of a new cholelitholytic agent, ursodeoxycholyl N-carboxymethylglycine and its esters in rats.

Abstract

Intestinal absorption, biliary excretion and metabolism of a calcium gallstone dissolving agent, [11,12-3H]ursodeoxycholyl-N-carboxymethylglycine (UDC-CMG) and its monoethyl, diethyl and dipivaloyloxyethyl esters (UDC-CMG-Et, UDC-CMG-Et2 and UDC-CMG-PV2) were studied in bile duct cannulated rats. Biliary recovery of [3H]-labeled UDC-CMG, UDC-CMG-Et and UDC-CMG-Et2 after intraduodenal administration were 65%, 80%, 98%, respectively. Radio-thin layer chromatography analysis of the bile revealed that UDC-CMG didn't undergo any biotransformation during administration and excretion. About 80% and 20% of radioactivity recovered in the bile was identified as UDC-CMG-Et and UDC-CMG, respectively, after intraduodenal administrations of [3H]UDC-CMG-Et2 and [3H]UDC-CMG-Et. The administered intact UDC-CMG-Et2 was not found in the bile. Intraduodenally administered [3H]UDC-CMG-PV2 was rapidly recovered in the bile. The total recovery rate was 78% within a 24 h period. More than 80% of the radioactivity recovered in the bile was found as UDC-CMG. Lesser amounts of the monopivaloyloxyethyl ester of UDC-CMG were also found, but intact UDC-CMG-PV2 was not detected in the bile as in the case of UDC-CMG-Et2. Among the esters of UDC-CMG investigated in the present studies, only UDC-CMG-PV2 was excreted in the bile mainly as the perhydrolyzed form, UDC-CMG. These results suggest the usefulness of UDC-CMG-PV2 as the pro-drug in calcium gallstone dissolution therapy.

• Authors: S Hatono, H Yoshida, M Matsunami, Y Ide, K Matsuda, T Yatsunami, T Fuwa, K Kihira, T Kuramoto, T Hoshita
• Journal: Journal of pharmacobio-dynamics (J Pharmacobiodyn) Vol. 14 Issue 10 Pg. 561-6 (Oct 1991) ISSN: 0386-846X [Print] Japan
• PMID: 1818097 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Tritium
• Ursodeoxycholic Acid
• ursodeoxycholyl N-carboxymethylglycine
• Calcium

Topics

• Animals
• Bile (metabolism)
• Calcium (metabolism)
• Cholelithiasis (drug therapy)
• Intestinal Absorption
• Male
• Rats
• Rats, Inbred Strains
• Tritium
• Ursodeoxycholic Acid (analogs & derivatives, pharmacokinetics, therapeutic use)