Abstract | OBJECTIVE: METHODS: An isolated, ventilated, ex vivo blood-perfused rabbit lung model was used. All groups underwent 2 hours of reperfusion after 18 hours of cold ischemia (4 degrees C). ATL-313 was administered 1 hour before ischemia intravenously, with the preservation solution, and/or during reperfusion. RESULTS: Both pretreatment of donor animals with ATL-313 or adding ATL-313 just during reperfusion improved pulmonary function, but significantly greater improvement was observed when pretreatment and treatment during reperfusion were combined (all P < .05). Myeloperoxidase levels, bronchoalveolar lavage tumor necrosis factor alpha levels, and pulmonary edema were all maximally decreased in the combined treatment group. The administration of an equimolar amount of the potent and highly selective adenosine 2A receptor antagonist, ZM 241385, along with ATL-313, resulted in the loss of protection conferred by ATL-313. CONCLUSIONS:
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Authors | Leo M Gazoni, Victor E Laubach, Daniel P Mulloy, A Bellizzi, Eric B Unger, Joel Linden, Peter I Ellman, Turner C Lisle, Irving L Kron |
Journal | The Journal of thoracic and cardiovascular surgery
(J Thorac Cardiovasc Surg)
Vol. 135
Issue 1
Pg. 156-65
(Jan 2008)
ISSN: 1097-685X [Electronic] United States |
PMID | 18179933
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- ATL 313
- Adenosine A2 Receptor Agonists
- Anti-Inflammatory Agents
- Piperidines
- Receptor, Adenosine A2A
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Topics |
- Adenosine A2 Receptor Agonists
- Animals
- Anti-Inflammatory Agents
(pharmacology, therapeutic use)
- Female
- In Vitro Techniques
- Lung Diseases
(prevention & control)
- Lung Transplantation
- Male
- Models, Animal
- Piperidines
(pharmacology, therapeutic use)
- Rabbits
- Receptor, Adenosine A2A
(drug effects, metabolism)
- Reperfusion Injury
(prevention & control)
- Tissue and Organ Harvesting
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