| Abstract | AIM: To confirm whether insulin regulates resistin expression and secretion during differentiation of 3T3-L1 preadipocytes and the relationship of resistin with insulin resistance both in vivo and in vitro. METHODS: Supernatant resistin was measured during differentiation of 3T3-L1 preadipocytes. L6 rat myoblasts and hepatoma cell line H4IIE were used to confirm the cellular function of resistin. Diet-induced obese rats were used as an insulin resistance model to study the relationship of resistin with insulin resistance. RESULTS: Resistin expression and secretion were enhanced during differentiation 3T3-L1 preadipocytes. This cellular differentiation stimulated resistin expression and secretion, but was suppressed by insulin. Resistin also induced insulin resistance in H4IIE hepatocytes and L6 myoblasts. In diet-induced obese rats, serum resistin levels were negatively correlated with insulin sensitivity, but not with serum insulin. CONCLUSION: Insulin can inhibit resistin expression and secretion in vitro, but insulin is not a major regulator of resistin in vivo. Fat tissue mass affects insulin sensitivity by altering the expression and secretion of resistin. |
| Authors | Feng Liu, Hong-Qi Fan, Jie Qiu, Bin Wang, Min Zhang, Nan Gu, Chun-Mei Zhang, Li Fei, Xiao-Qing Pan, Mei Guo, Rong-Hua Chen, Xi-Rong Guo
(Affiliation: Institute of Pediatrics of Nanjing Maternity and Child Health Hospital of the Nanjing Medical University, Nanjing 210004, Jiangsu Province, China.)
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| Journal | World journal of gastroenterology : WJG
(World J Gastroenterol)
Vol. 14
Issue 1
Pg. 95-100
(Jan 7 2008)
ISSN: 1007-9327 China |
| PMID | 18176969
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Hypoglycemic Agents
- RNA, Messenger
- Resistin
- Insulin
|
| Topics |
- 3T3-L1 Cells
- Adipocytes
(drug effects, metabolism, secretion)
- Animals
- Hypoglycemic Agents
(pharmacology)
- Insulin
(pharmacology)
- Insulin Resistance
(physiology)
- Male
- Mice
- Obesity
(metabolism)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Resistin
(genetics, metabolism, secretion)
|
