Abstract | PURPOSE: MATERIALS AND METHODS: RESULTS: The ingestion of BCG, h-kBCG, and E. coli did not affect the viability of the Mphi cells within 2 days. The phagocytosis caused generation of TNF-alpha and NO, being more significant with E. coli than with both types of BCGs. The phagocytosis of both types of BCGs stimulated the phagocytic uptake of cPLGA and PSL MS's, which took place prior to the generation of TNF-alpha or NO, but that of E. coli suppressed the uptake of both MS's. CONCLUSION: Mycobacterial infection stimulated the phagocytic uptake toward cPLGA MS. These results suggest that RFP-PLGA MS is favorable for overcoming tuberculosis.
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Authors | Keiji Hirota, Keishiro Tomoda, Hiroyuki Inagawa, Chie Kohchi, Gen-Ichiro Soma, Kimiko Makino, Hiroshi Terada |
Journal | Pharmaceutical research
(Pharm Res)
Vol. 25
Issue 6
Pg. 1420-30
(Jun 2008)
ISSN: 0724-8741 [Print] United States |
PMID | 18172577
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Tumor Necrosis Factor-alpha
- Polylactic Acid-Polyglycolic Acid Copolymer
- Polyglycolic Acid
- Nitric Oxide
- Lactic Acid
- Rifampin
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Topics |
- Animals
- Cell Survival
- Cells, Cultured
- Escherichia coli
(immunology)
- Lactic Acid
(administration & dosage)
- Macrophages, Alveolar
(immunology)
- Microspheres
- Mycobacterium
(immunology)
- Mycobacterium bovis
(immunology)
- Nitric Oxide
(biosynthesis)
- Phagocytosis
- Polyglycolic Acid
(administration & dosage)
- Polylactic Acid-Polyglycolic Acid Copolymer
- Rats
- Rifampin
(administration & dosage)
- Tumor Necrosis Factor-alpha
(biosynthesis)
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