Abstract | BACKGROUND: METHODS: To investigate the roles of gangliosides in breast tumor development, GM3 synthase was silenced in the highly metastatic 4T1 cells and over-expressed in the non-metastatic 67NR cells. The behavior of breast cancer cells was examined in vitro using migration assay, invasion assay, and soft agar assay. Tumor formation and metastasis in vivo were examined using a well established mouse mammary tumor model. RESULTS:
GM3 synthase silencing in 4T1 cells significantly inhibited cell migration, invasion and anchorage-independent growth in vitro, and lung metastasis in vivo. In addition, over-expression of GM3 synthase in nonmetastatic 67NR cells significantly induced cell migration and anchorage-independent growth. Further studies indicated that activation of the phosphoinositide-3 kinase/Akt pathway, and consequently inhibition of nuclear factor of activated T cell (NFAT)1 expression, could be the mechanism underlying the suppression of breast cancer migration/invasion induced by GM3 synthase silencing. CONCLUSION:
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Authors | Yuchao Gu, Junhua Zhang, Wenyi Mi, Jing Yang, Feng Han, Xinzhi Lu, Wengong Yu |
Journal | Breast cancer research : BCR
(Breast Cancer Res)
Vol. 10
Issue 1
Pg. R1
( 2008)
ISSN: 1465-542X [Electronic] England |
PMID | 18171481
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Sialyltransferases
- haematoside synthetase
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Topics |
- Animals
- Breast Neoplasms
(enzymology, genetics, pathology)
- Cell Line, Tumor
- Disease Models, Animal
- Female
- Lung Neoplasms
(enzymology, genetics, secondary)
- Mice
- Sialyltransferases
(biosynthesis)
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