Effect of dl-praeruptorin A on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes.

Abstract

BACKGROUND:

Intermediate filament (IF) proteins have been thought to play a role in nuclear centration, organelle movement and maintenance of cell shape. dl-praeruptorin A (Pd-Ia), a novel Ca2+-influx blocker and K+-channel opener isolated from Chinese traditional herbal medicine Qian-Hu, has been demonstrated to inhibit expression of apoptosis related proteins and reduce the level of proinflammatory factors in ischemia/reperfusion myocardiocytes. Morphologically, whether Pd-Ia effects myocardiocyte IFs remains unclear. The purpose of this study was, for the first time, to evaluate the in vivo effects of Pd-Ia on IF desmin and vimentin content in order to further explore its cardioprotection against ischemia and elucidate its mechanism of action.

METHODS:

Rats underwent a 30 minutes coronary occlusion followed by 120 minutes reperfusion. Assessment of desmin and vimentin content of myocardiocytes was performed by immunohistochemistry, Western blot, Hematoxylin-Eosin staining and densitometry.

RESULTS:

Pretreatment with i.v. infusion of Pd-Ia prior to ischemia significantly increased desmin and vimentin content and alleviated defects caused by the ischemia/reperfusion insult, e.g. with Pd-Ia at a dose of 0.5 or 1.0 mg/kg, integrated density values of desmin were increased from 61 478 +/- 10 074 to 177 408 +/- 10 395 and 195 784 +/- 20 057, and vimentin from 59 189 +/- 19 853 to 164 781 +/- 19 543 and 185 696 +/- 20 957 (P < 0.01, vs placebo), respectively. The recovery of desmin seemed to be stronger and appeared earlier than that of vimentin.

CONCLUSION:

Pd-Ia protectively increased IF desmin and vimentin content in ischemia/reperfusion myocardiocytes, which might be partially responsible for its cardioprotection against ischemia.

• Authors: He Chang, Xiao-yu Chu, Jun Zou, Tian-hui Chang
• Journal: Chinese medical journal (Chin Med J (Engl)) Vol. 120 Issue 24 Pg. 2256-9 (Dec 20 2007) ISSN: 0366-6999 [Print] China
• PMID: 18167213 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Coumarins
• Desmin
• Drugs, Chinese Herbal
• Vimentin
• praeruptorin A
• Calcium

Topics

• Animals
• Blotting, Western
• Calcium (metabolism)
• Coumarins (pharmacology)
• Desmin (analysis)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (pharmacology)
• Female
• Immunohistochemistry
• Male
• Myocardial Ischemia (drug therapy, metabolism)
• Myocardial Reperfusion Injury (prevention & control)
• Myocytes, Cardiac (chemistry, drug effects, pathology)
• Rats
• Rats, Wistar
• Vimentin (analysis)