Analgesic, anti-inflammatory and antioxidant properties of Buddleja globosa, Buddlejaceae.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Buddleja globosa, known as "matico", is employed in Chile for wound healing.

AIM OF THE STUDY:

To validate the traditional use of the crude drug through in vivo and in vitro evaluation of the anti-inflammatory, analgesic and antioxidant properties of its extracts.

MATERIALS AND METHODS:

Sequential hexane, dichloromethane, methanol and total methanol extracts were studied using bioguided fractionation. The following activities were investigated: analgesic (writhing test), oral and topic anti-inflammatory (paw- and ear-induced edema), free radical scavenging and antioxidant activities (1,1-diphenyl-2-picrylhydrazyl, DPPH, superoxide anion, lipid peroxidation and xanthine oxidase inhibition). Sodium naproxen, nimesulide, indomethacin were used as reference drugs for in vivo, quercetin and allopurinol for in vitro assays.

RESULTS:

A mixture of alpha- and beta-amyrins was isolated from the hexane extract that showed 41.2% of analgesic effect at 600 mg/kg, inhibited by 47.7 and 79.0% the arachidonic acid (AA) and 12-deoxyphorbol-13-decanoate (TPA)-induced inflammation at 3mg/20 microL/ear, respectively. A mixture of beta-sitosterol, stigmasterol, stigmastenol, stigmastanol and campesterol was isolated from the fraction CD4-N and beta-sitosterol-glycoside from the fraction CD5-N, reducing TPA-induced inflammation by 78.2 and 83.7% at 1mg/20 microL/ear, respectively. The fraction CD4-N at 300 mg/kg also showed analgesic activity (38.7%). The methanol extract at 600mg/kg per os showed anti-inflammatory effect (61.4%), topic anti-inflammatory (56.7% on TPA) and analgesic activity (38.5%). Verbascoside and luteolin-7-O-glucoside were the major components of the methanol extract; apigenin 7-O-glucoside was also detected. Inhibition of superoxide anion, lipoperoxidation, and DPPH bleaching effect was found in the methanol serial and global extracts.

CONCLUSIONS:

The present report demonstrate the analgesic and anti-inflammatory properties of Buddleja globosa and validate its use in Chilean traditional medicine.

• Authors: N Backhouse, L Rosales, C Apablaza, L Goïty, S Erazo, R Negrete, C Theodoluz, J Rodríguez, C Delporte
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 116 Issue 2 Pg. 263-9 (Mar 05 2008) ISSN: 0378-8741 [Print] Ireland
• PMID: 18164566 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Analgesics
• Anti-Inflammatory Agents
• Antioxidants
• Plant Extracts

Topics

• Analgesics (pharmacology)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (pharmacology)
• Buddleja (chemistry)
• Female
• Male
• Mice
• Plant Extracts (pharmacology)
• Spectrum Analysis