Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay.

Abstract

Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a (3)H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.

• Authors: Makito Tanaka, Yoshiyuki Takahashi, Yinyan Xu, Nao Yoshida, Ayami Yoshimi, Itzel Bustos Villalobos, Asahito Hama, Nobuhiro Nishio, Hirokazu Hidaka, Yue Wang, Hiroshi Yagasaki, Seiji Kojima
• Journal: Leukemia research (Leuk Res) Vol. 32 Issue 7 Pg. 1036-42 (Jul 2008) ISSN: 0145-2126 [Print] England
• PMID: 18164384 (Publication Type: Journal Article)

Chemical References

• Granulocyte-Macrophage Colony-Stimulating Factor
• PTPN11 protein, human
• Protein Tyrosine Phosphatase, Non-Receptor Type 11
• Thymidine

Topics

• Cell Proliferation
• Child, Preschool
• Female
• Genes, ras
• Granulocyte-Macrophage Colony-Stimulating Factor (pharmacology)
• Humans
• Infant
• Leukemia, Myelomonocytic, Juvenile (genetics, metabolism, pathology)
• Male
• Protein Tyrosine Phosphatase, Non-Receptor Type 11 (genetics)
• Thymidine (metabolism)