Abstract |
Interstitial cystitis (IC) is a chronic inflammatory condition of the urinary bladder with a strong autoimmune component. Currently, the major challenge in IC treatment is the development of effective therapies. RDP58 is a novel d- amino acid decapeptide with potent immunosuppressive activity. In this study, we investigated whether RDP58 was effective as an intravesical agent for treating bladder autoimmune inflammation in a transgenic mouse model (URO-OVA mice). URO-OVA mice were adoptively transferred with syngeneic activated splenocytes of OT-I mice transgenic for the OVA-specific CD8(+) TCR for cystitis induction and treated intravesically with RDP58 at days 0 and 3. Compared with controls, the RDP58-treated bladders showed markedly reduced histopathology and expressions of mRNAs and proteins of TNF-alpha, NGF and substance P. To determine whether the inhibition of bladder inflammation by RDP58 was due to the interference with effector T cells, we treated the cells with RDP58 in vitro. Cells treated with RDP58 showed reduced production of TNF-alpha and IFN-gamma as well as apoptotic death. Collectively, these results indicate that RDP58 is effective for treating T cell-mediated experimental autoimmune cystitis and may serve as a useful intravesical agent for the treatment of autoimmune-associated bladder inflammation such as IC.
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Authors | Wujiang Liu, Barry R Deyoung, Xiaohong Chen, David P Evanoff, Yi Luo |
Journal | Journal of autoimmunity
(J Autoimmun)
Vol. 30
Issue 4
Pg. 257-65
(Jun 2008)
ISSN: 0896-8411 [Print] England |
PMID | 18162370
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Cytokines
- Immunosuppressive Agents
- Peptides
- allotrap
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Topics |
- Animals
- Autoimmune Diseases
(drug therapy, immunology, pathology)
- CD8-Positive T-Lymphocytes
(drug effects, immunology)
- Cystitis
(drug therapy, immunology, pathology)
- Cytokines
(biosynthesis, drug effects)
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- Female
- Flow Cytometry
- Immunosuppressive Agents
(administration & dosage)
- Injections, Intralesional
- Mice
- Mice, Transgenic
- Peptides
(administration & dosage)
- Reverse Transcriptase Polymerase Chain Reaction
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