Effects of a Shuangling Fuzheng anticancer preparation on the proliferation of SGC-7901 cells and immune function in a cyclophosphamide-treated murine model.

Abstract

AIM:

To study the inhibitory effects of a Shuangling Fuzheng anticancer preparation (SFAP) on the human gastric cancer cell line SGC-7901 in vitro as well as its immune-modulated effects in a cyclophosphamide-treated murine model.

METHODS:

MTT experiments and immunocytochemistry ABC experiments were performed for detecting the proliferation of SGC-7901 cells in vitro and protein expression of c-myc. The staphylococcal protein A (SPA) rosette test was utilized for measuring the ratio of T-lymphocyte subsets from peripheral blood in a cyclophosphamide-treated murine model. Enzyme-linked immunosorbant assay (ELISA) was performed for measuring the levels of serum sIL-2R in treated mice, while immunoturbidimetry was used for measuring the levels of immunoglobulins (Ig).

RESULTS:

SFAP (40-640 mg/L, 48 h) inhibited the proliferation of SGC-7901 cells, and a positive correlation was noted between inhibitory effects and dosage. At a dosage of 160-320 mg/L in cultured cells, the expression of c-myc was decreased. SFAP (50-200 mg/kg) increased the percentage of CD3+ and CD4+ T-lymphocytes, the ratio of CD4/CD8, and the contents of Ig such as IgM, IgG or IgA, but decreased the levels of serum sIL-2R in peripheral blood from cyclophosphamide-treated mice.

CONCLUSION:

SFAP can inhibit the proliferation of SGC-7901 cells via the c-myc gene. In addition, SFAP can modulat the cellular and humoral immunity in cyclophosphamide-induced immunosuppressed mice.

• Authors: Hua-Sheng Chen, Jue Chen, De-Li Cui, Yuan-Yuan Zheng, Ai-Hua Xu, Gang Chen, Ling-Chang Jia
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 13 Issue 48 Pg. 6575-80 (Dec 28 2007) ISSN: 1007-9327 [Print] United States
• PMID: 18161930 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Drugs, Chinese Herbal
• Immunoglobulins
• Immunosuppressive Agents
• MYC protein, human
• Proto-Oncogene Proteins c-myc
• Receptors, Interleukin-2
• Shuangling Fuzheng anticancer preparation
• Cyclophosphamide

Topics

• Animals
• Antibody Formation (drug effects)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cells, Cultured
• Cyclophosphamide (pharmacology)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (pharmacology)
• Female
• Humans
• Immunity, Cellular (drug effects)
• Immunoglobulins (blood)
• Immunosuppression Therapy
• Immunosuppressive Agents (pharmacology)
• Mice
• Mice, Inbred ICR
• Proto-Oncogene Proteins c-myc (metabolism)
• Receptors, Interleukin-2 (blood)
• Stomach Neoplasms (metabolism, pathology)
• T-Lymphocyte Subsets (metabolism, pathology)