Abstract | PURPOSE: METHODS: Clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) (n = 4), methicillin-sensitive S. aureus (MSSA) (n = 8), methicillin-resistant Staphylococcus epidermidis (MRSE) (n = 8), Pseudomonas aeruginosa (n = 4) and Escherichia coli (n = 4) were tested in duplicate. Bacterial suspensions of each isolate were made in a control solution of normal saline and Mueller-Hinton broth (MHB), and in a lock solution of ethanol 30%, TSC 4% and MHB. Suspensions were incubated at 37 degrees C for 48 h. Colony counts were determined from samples collected at t = 0 h (before exposure to the ethanol/ TSC lock), t = 1 h (one hour after exposure to the ethanol/ TSC lock), t = 24 h and t = 48 h. To confirm the absence of viable organisms in the lock solution, the remaining volume at 48 h was filtered through a 0.45 microm filter. The filter was rinsed with 15 mL sterile water and plated on tryptic soy agar ( TSA). RESULTS: All controls demonstrated significant growth over 48 h. In the lock solutions, initial inocula were reduced to 0 viable colonies by t = 1 h (6-log kill), and there was no growth at t = 24 and 48 h. Filtering of lock solutions also showed no growth. These results were consistent among duplicates of all isolates. CONCLUSIONS: The 30% ethanol/4% TSC lock solution consistently eradicated MRSA, MSSA, MRSE, P. aeruginosa and E. coli within 1 h of exposure. Experiments are currently underway to test this novel lock solution on preventing biofilm production by these pathogens.
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Authors | T A Takla, S A Zelenitsky, L M Vercaigne |
Journal | The journal of vascular access
(J Vasc Access)
2007 Oct-Dec
Vol. 8
Issue 4
Pg. 262-7
ISSN: 1129-7298 [Print] United States |
PMID | 18161672
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Bacterial Agents
- Citrates
- trisodium citrate
- Ethanol
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Topics |
- Anti-Bacterial Agents
(pharmacology)
- Bacterial Infections
(etiology, microbiology, prevention & control)
- Catheters, Indwelling
(microbiology)
- Citrates
(pharmacology)
- Colony Count, Microbial
- Escherichia coli
(drug effects, growth & development)
- Ethanol
(pharmacology)
- Methicillin Resistance
- Pseudomonas aeruginosa
(drug effects, growth & development)
- Renal Dialysis
(adverse effects, instrumentation)
- Staphylococcus aureus
(drug effects, growth & development)
- Staphylococcus epidermidis
(drug effects, growth & development)
- Time Factors
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