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Induction of apoptosis in HT-29 colon cancer cells by phloretin.

Abstract
Phloretin, which is present in apples and pears, has been found to inhibit the growth of several cancer cells and induce apoptosis of B16 melanoma and HL60 human leukemia cells. The present study examined whether and how phloretin induces apoptosis of HT-29 human colon cancer cells. Phloretin (0-100 micromol/L) substantially decreased viable cell number and induced apoptosis of HT-29 cells in a dose-dependent manner. Western blot analysis of total cell lysates revealed that phloretin increased the protein levels of Bax but had no effect on Bcl-2. In addition, phloretin induced cleavage of caspase-8, -9, -7, and -3 and poly(ADP-ribose) polymerase. Furthermore, phloretin increased the levels of cytochrome c and Smac/Diablo in the cytosol. The present results indicate that phloretin inhibits HT-29 cell growth by inducing apoptosis, which may be mediated through changes in mitochondrial membrane permeability and activation of the caspase pathways.
AuthorsSo Young Park, Eun Ji Kim, Hyun-Kyung Shin, Dae Young Kwon, Myung Sunny Kim, Young-Joon Surh, Jung Han Yoon Park
JournalJournal of medicinal food (J Med Food) Vol. 10 Issue 4 Pg. 581-6 (Dec 2007) ISSN: 1096-620X [Print] United States
PMID18158826 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • bcl-2-Associated X Protein
  • Caspases
  • Phloretin
Topics
  • Apoptosis (drug effects)
  • Blotting, Western
  • Caspases (metabolism)
  • Cell Division (drug effects)
  • Enzyme Activation (drug effects)
  • HT29 Cells
  • Humans
  • Phloretin (pharmacology)
  • bcl-2-Associated X Protein (analysis)

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